早期肌肉特异性mRNA在骨髓干细胞的表达  

Expression of early muscle specific mRNA in bone marrow stem cells

作　　者：曾缨[1] 张成[1] 李才明[1] 柳太云[1] 

机构地区：[1]中山大学附属第一医院神经科,广东广州510080

出　　处：《第四军医大学学报》2006年第18期1637-1640,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金项目(30370510);广东省自然科学基金项目(31693);卫生部临床重点项目(2001321)

摘　　要：目的:研究早期肌肉特异性mRNA在骨髓干细胞中的特异性表达.方法:利用RT-PCR研究不同时期C57鼠和不同代龄SD大鼠骨髓干细胞早期肌肉特异性mRNA(包括MyoD,Myf5,myogen in,MRF4和desm in)的表达.结果:Myf5及desm in在新鲜分离、培养了2 d和7 d的悬浮与贴壁的C57小鼠骨髓干细胞中均有表达,没有检测到MyoD,myogen in和MRF4在此时期小鼠骨髓干细胞的表达.MyoD与desm in在原代至第6代SD大鼠骨髓间质干细胞中均有表达,没有检测到myogen in和MRF4在各代细胞中的表达.结论:骨髓干细胞能表达一定水平的早期肌肉特异性mRNA.AIM： To study the expression of early muscle specific mRNA in bone marrow stem cells. METHODS： The expression of early muscle specific mRNA, including MyoD, Myf5, myogenin, MRF4 and desmin, in bone marrow stem cells of C57 mice of different phases and SD rats of different generations was detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. RESULTS ： In different cultured phases （ day 0,2,7 ） of C57 mouse bone marrow stem cells, RT-PCR analysis showed the presence of Myf5 and desmin, whereas MyoD, myogenin and MRF4 were not detected. In different generations （from the primary to the 6th generation） of rat bone marrow mesenchymal stem cells, MyoD and desmin were detected, whereas myogenin and MRF4 were not detected by RT-PCR analysis. CONCLUSION： Bone marrow stem cells can express early muscle specific mRNA to some extent.

关 键 词：骨髓祖代细胞 组织特异性 肌形成调节因子 

分 类 号：R654.2[医药卫生—外科学]