大鼠脊髓背根神经节内神经前体细胞的观察  

Observation of neural precursor cells in rat dorsal root ganglia

作　　者：宋嵬[1] 曾水林[1] 朱建宝[1] 王磊[1] 李升[1] 阎荣[1] 李凤飞[1] 

机构地区：[1]东南大学神经生物学研究所,江苏南京210009

出　　处：《第四军医大学学报》2006年第18期1679-1682,共4页Journal of the Fourth Military Medical University

摘　　要：目的:探讨大鼠脊髓背根神经节(DRG)内是否存在神经前体细胞.方法:取成年和胚胎大鼠DRG冰冻切片,进行B rdu,Nestin,P75免疫荧光组织化学检测,分别取成年和胚胎大鼠DRG进行原代细胞培养,观察其增殖与分化情况,并行Nestin免疫组织化学和B rdu,P75,NF,GFAP免疫荧光组织化学鉴定.结果:在成年和胚胎大鼠DRG冰冻切片上均见有B rdu/Nestin和Nestin/P75免疫荧光双标细胞.细胞培养观察到取自DRG的细胞有分裂增殖,呈Nestin免疫组织化学阳性反应和B rdu,P75免疫荧光组织化学阳性反应;培养第三代细胞加入胎牛血清后出现分化,分化细胞呈NF和GFAP免疫荧光组织化学阳性反应.结论:大鼠DRG内存在有分裂增殖的神经前体细胞,这些细胞可以分化为神经元和神经胶质细胞.AIM： To investigate neural precursor cells in ra dorsal root ganglia （DRG）. METHODS： DRG from embryonic and adult rats were frozen and sliced for Brdu, Nestin, p^75 immunofluoreseenee histoehemieal test. Additionally, the cells of ganglia were respectively cultured and observed in thei proliferation and differentiation. The cells were identified by Brdu, Nestin, p^75, NF and GFAP immunofluoreseenee histo. chemical test, RESULTS： In the frozen DRG sections from adult and embryonic rats, Brdu/Nestin and Nestin/P^75 positive eelh were found. The cultured cells could divide and proliferate am showed Nestin, Brdu, p^75 positive, The third-passage cells could differentiate in the presence of fetal bovine serum, The differentiating cells showed NF and GFAP positive. CONCLUSION： There are neural precursor cells that can divide and proliferate in rat DRG. They can differentiate into neuron and glial cells.

关 键 词：大鼠 神经前体细胞 神经节 脊 细胞培养技术 免疫荧光组织化学 

分 类 号：Q24[生物学—细胞生物学]