Interaction of APT with BSA or HSA  

作　　者：CUI Fengling CUI Yanrui LUO Hongxia YAO Xiaojun FAN Jing LU Yan 

机构地区：[1]Henan Key Laboratory for Environmental Pollution Control, School of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China [2]Department of Chemistry, Renmin University of China, Beijing 100872, China [3]Department of Chemistry, Lanzhou University, Lanzhou 730000, China

出　　处：《Chinese Science Bulletin》2006年第18期2201-2207,共7页

基　　金：the Young Backbone Teacher Support Plan of Henan Colleges and Universities （Grant No. 200470）; the Department of Education of Henan Province （Grant No. 2006150012）; the National Natural Science Foundation of China （Grant Nos. 20443002 , 20575077）.

摘　　要：In this work, N-n-amyl-N’-(sodium p- aminobenzenesulfonate) thiourea (APT) containing saturated fatty hydrocarbon group was synthesized. Fluorescence quenching methods in combination with UV absorption spectra and molecule modeling method were used to study the interaction between APT and bovine serum albumin (BSA) or human se-rum albumin (HSA). The binding constants of APT with BSA or HSA were determined at different tem-peratures under the optimum conditions based on the fluorescence quenching results. The binding characteristics of APT and BSA or HSA were reported and the binding sites were obtained. The binding mode was suggested to be mainly hydrophobic interaction, which was consistent with molecular modeling study.In this work, N-n-amyl-N＇-（sodium p- aminobenzenesulfonate） thiourea （APT） containing saturated fatty hydrocarbon group was synthesized. Fluorescence quenching methods in combination with UV absorption spectra and molecule modeling method were used to study the interaction between APT and bovine serum albumin （BSA） or human serum albumin （HSA）. The binding constants of APT with BSA or HSA were determined at different temperatures under the optimum conditions based on the fluorescence quenching results. The binding characteristics of APT and BSA or HSA were reported and the binding sites were obtained. The binding mode was suggested to be mainly hydrophobic interaction, which was consistent with molecular modeling study.

关 键 词：APT BSA 脂肪族烃 牛血清蛋白 HSA 人血清白蛋白 荧光光谱 分子模型 

分 类 号：Q51[生物学—生物化学]