2例猪弓形虫病的特异PCR诊断与虫株分离  

Diagnosis of Swine Toxoplasmosis by Specific PCR Assay and the Isolation of Toxoplasma gondii Strains

作　　者：廖申权[1] 翁亚彪[1] 宋慧群[1] 杨傲冰[2] 崔建鑫 张翰[1] 朱兴全[1] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]广东省农业科学院兽医研究所,广州510640 [3]惠州市畜牧兽医科学研究所,惠州516003

出　　处：《热带医学杂志》2006年第9期969-971,共3页Journal of Tropical Medicine

基　　金：广东省自然科学基金团队项目(No.05200638);广东省科技计划项目(No.2004B20201008);国家杰出青年科学基金(No.30225033)。

摘　　要：目的应用特异PCR方法诊断猪弓形虫病并通过动物接种的方法分离弓形虫虫株。方法用涂片镜检、特异PCR方法检测病料,用经PCR方法检测为阳性的病料接种小白鼠,经连续传代分离弓形虫虫株。结果病例1的肺及肺门淋巴结涂片镜检见有少量的弓形虫滋养体,但病例2的颌下淋巴结及肺门淋巴结涂片镜检未见有弓形虫滋养体。用特异PCR方法检测两个病例的上述病料,均得到弓形虫的特异条带。小白鼠接种试验从每个病例均分离出一个弓形虫虫株。结论特异PCR诊断方法能较快速、准确地检测猪弓形虫病,小白鼠接种试验是一种较好的弓形虫虫株分离方法。Objective To diagnose swine toxoplasmosis by specific PCR assay and to isolate Toxoplasma gondii strain from infected tissues through/noculation in mice. Methods Swine toxoplasmosis was diagosed by microscopy of tissue smear amd specific PCR assay （using the first internal transcribed spacer of ribosomal DNA as genetic marker）. The diagnosis was further confrimed and the T. gondii strain was isolated by the inoculation of infected tissues in mice. Results Two cases of swine toxoplasmosis were analyzed. A few T. goadii tachyzoites were observed in case 1 by microscopy, but not in case 2. In both cases, DNA fragment specific for T. gondii was amplified from genomic DNA from infected tissues by specific PCR assay. One strain of T. gondii was isolated from each case through inoculation of infected tissues and serial passages in mice. Conclusion Specific PCR assay is a rapid and accurate method for diagnosing swine toxoplasmosis. Inoculation of infected tissues in mice is an effective method for the isolation of T.gondii strains and to confirm the diagnosis.

关 键 词：弓形虫 猪弓形虫病 特异PCR 

分 类 号：R531.8[医药卫生—内科学]