氧化铝羟基磷灰石人工骨与兔骨的生物相容性观察  

Biocompatibility between aluminum oxide hydroxyapatite artificial bone and rabbit bone

作　　者：赵燚[1] 徐武清[1] 赵喆[2] 马英峰[1] 赵自强[1] 

机构地区：[1]宁夏医学院,宁夏回族自治区银川市750004 [2]瑞典斯德哥尔摩大学

出　　处：《中国临床康复》2006年第37期57-60,i0003,共5页Chinese Journal of Clinical Rehabilitation

基　　金：宁夏自然科学基金资助项目(E006-2004);宁夏国际合作资助项目(05GJ-11400)~~

摘　　要：目的:评价致密氧化铝-羟基磷灰石人工骨与兔骨的生物相容性。方法:人工骨实验材料2004-12于瑞典斯德哥尔摩大学阿伦尼乌斯实验室烧结成形;动物实验于2005-03/12在宁夏医学院中医学院实验中心完成。①采用放电等离子烧结技术制备致密氧化铝-羟基磷灰石人工骨。②选健康家兔12只,分为2组,空白对照组和氧化铝/羟基磷灰石人工骨组,后者根据两种物质的体积分数又分为3个亚组。0.4/0.6组4只,植入氧化铝/羟基磷灰石为0.4/0.6材料;0.5/0.5组4只,植入氧化铝/羟基磷灰石为0.5/0.5材料;致密纯羟基磷灰石组4只,植入氧化铝/羟基磷灰石为0/1材料。上述3组均于右后肢植入相应人工骨材料;空白对照组取致密纯羟基磷灰石组左后肢4个肢体,只钻凿方形孔或钻圆形孔骨缺损,不植入实验材料。③术后1d,4,8,12周通过一般情况观察、大体解剖观察、CT摄片、扫描电镜观察人工骨与兔骨结合状况。结果:12只家兔均进入结果分析。①家兔术后一般情况:术后1周手术伤口全部愈合,伤口不肿,无分泌物,无窦道形成。②家兔骨缺损区及植入材料大体解剖观察结果:术后4周,植入3种材料与兔骨紧密结合,材料表面较多软/硬骨痂形成,骨膜覆盖。8,12周,材料被骨痂完全包埋,材料与宿主骨之间形成紧密结合层。术后各时期,材料周围软组织未见变性、坏死及包囊。③CT观察结果:术后12周,0.4/0.6组和0.5/0.5组,人工骨与骨皮质发生融合呈骨性连接,与人工骨结合处皮质增厚形成纺锤形。与纯羟基磷灰石组无明显区别。④扫描电镜观察结果:术后12周,取0.4/0.6组、0.5/0.5组氧化铝-羟基磷灰石材料与宿主骨已紧密结合无间隙存在,材料与骨质的界面呈现融合,与致密羟基磷灰石对照组比较无明显差别。结论:放电等离子技术烧结的氧化铝-羟基磷灰石人工骨有良好的生物相容性,可作为进一步研究的实验�ATM： To evaluate the biocompatibility between fully dense aluminum oxide hydroxyapatite （AO-HA） artificial bone and rabbit bone. METHODS： Artificial bone materials were prepared in Arrhenius Laboratory, Stockholm University by sintering; and the experiment was conducted in the experimental center of Department of Traditional Chinese Medicine, Ningxia Medical College from March to December 2005. ①The fully dense AO-HA artificial bone was made by Spark Plasma Sintering technique. ②Twelve healthy rabbits were selected and divided into 2 groups： control group and AO-HA artificial bone group. The latter group was subdivided into 3 subgroups according to the volume fraction of the two matters： 0.4/0.6 group implanted 0.4/0.6 AO-HA materials; 0.5/0.5 group implanted 0.5/0.5 AO-HA materials and fully dense HA group implanted 0/1 AO-HA materials with 4 rabbits in each subgroup. All the artificial bone material was implanted into the right hind limbs of the 3 subgroups; 4 left limbs of the fully dense HA group was drilled bone defects with square or round holes not implanting any material, which served as the HA control group. ③Scanning electron microscope （SEM）, CT and X-ray were applied after operation of 1 day, 4, 8 and 12 weeks, respectively to observe the general and gross conditions of integration between the artificial bone and rabbit bone. RESULTS： All the 12 rabbits were involved in the result analysis. ① General condition of rabbits after operation： One week after operation, the wound was healed without swelling; secretion and sinus tract.②Gross anatomic observation of defects and implanted material： After 4 weeks of operation, the 3 kinds of implanted materials were closely integrated with rabbit bone, and many soft/hard bone callus were found on the material surface covered with periost. After 8 and 12 weeks, materials were embedded by bone callus, the close conjugated layer was formed between material and host bone. No degeneration, necrosis or encyst was found in the surroun

关 键 词：氧化铝 羟基磷灰石类 骨代用品 生物相容性材料 

分 类 号：R318[医药卫生—生物医学工程]