重组人生长激素对老化皮肤胶原蛋白代谢的作用  

Effect of recombinant human growth hormone on the collagen metabolism of senile skins

作　　者：李卫[1] 亓发芝[2] 高成金[1] 

机构地区：[1]复旦大学附属金山医院烧伤整形科,上海市200540 [2]复旦大学附属中山医院整形外科,上海市200032

出　　处：《中国临床康复》2006年第37期78-80,i0004,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨哺乳动物细胞源性生长激素抗皮肤老化的作用机制。方法:实验于2004-03/12在复旦大学附属金山医院动物实验室完成。选择18～20月龄健康SD大鼠90只,随机数字表法分为3组,重组人生长激素0.08U/kg组,重组人生长激素0.12U/kg组,生理盐水对照组,每组30只。每组又分为用药前及用药后4,6,8,10,12周6个时间点,每个时间点5只。重组人生长激素0.08,0.12U/kg组:0.02U/mL重组人生长激素悬液分别行皮下注射0.08,0.12U/kg,3次/周,共12周。生理盐水对照组:以生理盐水皮下注射。另选取10只2～3月龄正常SD大鼠为空白对照组。于各时间点切取大鼠背部标记区的皮肤组织,行Masson染色并测定皮肤胶原厚度,反转录-聚合酶链反应检测皮肤转化生长因子βⅠ型和Ⅱ型受体mRNA的表达。结果:纳入动物100只,均进入结果分析。①大鼠皮肤胶原厚度:重组人生长激素0.08U/kg组注射8周胶原开始增厚,重组人生长激素0.12U/kg组第6周胶原开始增厚,厚度明显大于生理盐水对照组犤分别为(787±18),(766±20),(649±50)μm,P<0.05犦,与空白对照组比较差异无显著性意义(P>0.05)。②重组人生长激素0.08,0.12U/kg组转化生长因子βⅠ型和Ⅱ型受体mRNA的表达高于生理盐水对照组(以注射4周为例,转化生长因子βⅠ型受体分别为0.321±0.011,0.534±0.013,0.000±0.000;转化生长因子βⅡ型受体分别为0.297±0.009,0.541±0.025,0.000±0.000,P<0.05)。结论:重组人生长激素可上调皮肤转化生长因子βⅠ型和Ⅱ型受体mRNA的表达,促进胶原增殖而发挥抗皮肤老化作用。AIM： To investigate the action mechanism of recombinant human growth hormone （rhGH） on the collagen metabolism of senile skins. METHODS： The experiment was conducted in the Animal Laboratory of Jinshan Hospital, Fudan University from March to December 2004. Ninety healthy 18-20-month-old SD rats were selected and randomly divided into 3 groups： 0.08 U/kg rhGH group, 0.12 U/kg rhGH group and saline control group with 30 rats in each group. Every group was subdivided into 6 subgroups according to 6 time points： before and 4, 6, 8, 10 and 12 weeks after administration with 5 rats at each time point. The 0.08 U/kg and 0.12 U/kg rhGH groups： The rats were injected subcutaneously 0.08 and 0.12 U/kg rhGH （0.02 U/mL） suspension, respectively, three times a week for 12 weeks. The saline control group： The rats were injected. Another ten normal 2-3-month-old SD rats were selected as blank control group. The skin graft was incised from each rat for Masson staining and the thickness of collagen was detected. RT-PCR was used to detect the expression of transforming growth factor of TβR Ⅰ and TβR Ⅱ respectively. RESULTS; All the 100 rats were involved in the result analysis. ①Collagen thickness： From the 8th week, the collagen of the 0.08 U/kg rhGH group began to thicken, and the collagen of the 0.12 U/kg rhGH group began to thicken from the 6th week, which were higher than the saline control group [（787±18）, （766±20）, （649±50）μm, P 〈 0.05], and had no significant difference compared with the blank control group （P 〉 0.05）. ②The expression of TβR Ⅰ and TβR Ⅱ of 0.08 and 0.12 U/kg rhGH groups were higher than the saline control group （at the 4^th week： TβR Ⅰ 0.321±0.011, 0534±0.013, 0.000±0.000; TβR Ⅱ 0.297±0.009, 0.541±0.025, 0.000±0.000, P 〈 0.05）. CONCLUSION： rhGH can accelerate the collagen proliferation by upregulating the expressions of TβR Ⅰ and TβR Ⅱ in the skin to play a key role in the anti-aging process.

关 键 词：皮肤衰老 生长激素 胶原 

分 类 号：R347.511[医药卫生—基础医学]