外源DNA导入糯玉米自交系D_0代变异植株过氧化物酶同工酶分析  

Study on the isoenzyme of the variant plants derived from exogenous DNA introduction into the inbreds of glutinous maize

作　　者：庾韦花[1] 张慧英[2] 韦家川[2] 吴子恺[2] 郝小琴[2] 王小敏[3] 

机构地区：[1]广西农业科学院,广西南宁530007 [2]广西大学农学院,广西南宁530005 [3]玉林师范学院,广西玉林537000

出　　处：《广西植物》2006年第5期510-512,共3页Guihaia

基　　金：广西自然科学基金项目(桂科基0236005);广西教育厅项目(桂教科研[2002]316)~~

摘　　要：采用改良浸种法分别将甘蔗总DNA与孟加拉超甜玉米自交系总DNA导入糯玉米自交系12-9-10,在D0代分别获得变异植株,采用聚丙烯酰胺垂直电泳法对该植株及供受体进行了过氧化物酶同工酶分析,其结果表明植株D0(糯×甘300)的过氧化物酶同工酶在迁移率Rf5=0.33和Rf6=0.40分别出现一条供体特有而受体没有的酶带,其酶带强弱与供体相同;在迁移率Rf8=0.61出现一条供受体都没有的弱带。植株D0(糯×孟甜300)-2在迁移率Rf6=0.59增加了一条供受体都没有的弱带。说明了外源DNA(或DNA片段)已转移到糯玉米自交系12-9-10中,并得到了表达。Through the improved methods of soaking emgryo,the total DNA of sugarcane and inbreds of sweet maize was successfully introduced into the inbreds of glutinous maize 12-9-10 respectively. And the variant plants were obtained from Do generation. We had analyzed the zymograms of the peroxidase isozyme by PAGE. The results showed that a peroxidase-isozyme strip which was of donor but not of receptor appeared at Rf5 =0.33 and Rf6 =0.40 of the plant D0（waxy×sug300） ,a peroxidase-isozyme weak strip which was not of donor and receptor appeared at Rf8 =0.61. For the plant D0（waxy×mta00）-2 ,a peroxidase-isozyme strip which was not of donor and receptor appeared at Rf6 = 0.59. Thus,it was confirmed that exogenous genes（or DNA seg- ments）were introduced into glutinous maize 12-9-10 and on expression.

关 键 词：浸种法 外源DNA 变异 过氧化物酶同工酶 

分 类 号：Q943[生物学—植物学]