尖吻蝮蛇毒小分子多肽的分离及抗血小板聚集作用  

Purification of a small peptide to inhibit platelet aggregation from Agkistrodon acutus venom

作　　者：雷丹青[1] 周先丽[1] 李映新[1] 

机构地区：[1]广西医科大学蛇毒研究所,广西南宁530021

出　　处：《中国药理学通报》2006年第9期1126-1129,共4页Chinese Pharmacological Bulletin

基　　金：广西壮族自治区自然科学基金资助项目(No0575062)

摘　　要：目的从尖吻蝮蛇毒中分离纯化一种抗血小板聚集小分子多肽,研究其理化性质以及对ADP、胶原、凝血酶诱导的血小板聚集反应的影响。方法经SephadexG-75凝胶过滤,超滤,DEAE-SepharoseCL-6B离子交换层析法分离纯化蛇毒组分,采用高效液相鉴定纯度,用SDS-凝胶电泳(SDS-PAGE)测定其分子量,用比浊法测定其抗血小板聚集活性。结果从尖吻蝮蛇毒中分离相对分子质量约为7862u等电点为4.29的组分,该组分能抑制由ADP、胶原、凝血酶诱导的血小板聚集并成剂量依赖性。结论此法成功地从尖吻蝮蛇毒中纯化出抗血小板聚集组分。该组分与去整合素比较相似,可能属于去整合素家族。Aim To purify a small platide inhibiting platelet aggregation from Agkistrodon Acutus snake venom, and to identify its physical and chemical properties and its effects on the platelet aggregation stimulated by ADP, collagen, and thrombin. Methods Extract snake venom through Superdex 75 gel filtration, ultrafiltration and DEAE-Sepharose CL-6B ionexchange. The purified product was identified by HPLC C18. The molecular weight was determined by SDS-polyacrylamid gel electrphoresis. Platelet aggragation was measured by nephelometry. Results The molecular weight of the peptide was 7 862 u and its purified from Agkistrodon Acutus snake venom is oelectric point was pH 4.29. This peptide dose-dependently inhibited the platelet aggregation induced by ADP, collagen, and thrombin. Conclusion The method has been proved to be successful for the purification of low molecular weight peptide fraction that inhibits platelet aggregation.

关 键 词：尖吻蝮蛇毒 分离提纯 血小板聚集抑制剂 

分 类 号：R331.124[医药卫生—人体生理学] R341.6[医药卫生—基础医学]