乌头碱中毒后脑神经细胞凋亡的实验研究  

Apoptosis in brain nerve cells of aconitine poisoning rats

作　　者：雷怀成[1] 向文采[1] 石亮[1] 

机构地区：[1]郧阳医学院,湖北十堰442000

出　　处：《山东医药》2006年第27期21-22,共2页Shandong Medical Journal

基　　金：湖北省教育厅科学研究计划指导性项目(2001C66)

摘　　要：目的观察乌头碱中毒后不同时间段内脑损害程度,以期为法医学鉴定提供形态学依据。方法用35只W istar大鼠制作乌头碱中毒模型,通过HE染色及凋亡细胞检测技术对乌头碱中毒后大鼠脑的病理变化进行系统研究,并将研究结果进行计算机病理学图像分析。结果大鼠在染毒后的不同时间段内,脑神经细胞凋亡数均高于正常对照组(P<0.05),且细胞凋亡数在染毒后不同时间段亦不相同(P均<0.05)。结论乌头碱中毒在临床症状不明显及毒物分析难以检测的情况下,应用凋亡细胞检测技术可成功检测出脑神经细胞病理学变化。[Objective] To observe pathological changes and apoptosis in rat brain nerve cells after aconitine poisoning. [Methods] Experimental models of 35 Wistar rats aconitine poisoning were established , and the technology of apoptosis detection was used. The regular HE staining and immunohistochemical staining results were analyzed by.computer image analysis system. [Results] Apoptosis never cells counts in poisoning group were higher than those of the control nomal group at different stages. In addition ,the apoptosis cells counts were different after different poisoning time in the same organ （ P 〈 0.05）. [Conclusion] Aconitine poisoning can not be detected by clinical symptoms or poison analysis while pathological changes can be found through the apoptosis detection.

关 键 词：乌头碱 中毒 细胞凋亡 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]