基于RAPD标记进行水稻籼粳分类研究  

Classification of Rice Cultivars with RAPD Molecular Markers

作　　者：姜树坤[1] 钟鸣[1] 徐正进 张丽[1] 马慧[1] 刘少霞[1] 

机构地区：[1]沈阳农业大学辽宁省农业生物技术重点实验室,沈阳110161 [2]辽宁省北方粳稻育种重点实验室,沈阳110161

出　　处：《沈阳农业大学学报》2006年第4期639-641,共3页Journal of Shenyang Agricultural University

基　　金：辽宁省博士启动基金(2001102061)

摘　　要：应用RAPD标记方法对30个水稻栽培品种进行了籼粳分类研究。从80个随机引物中筛选出了扩增出具有多态性的谱带的18个引物,并从中选出重复性高、稳定性好的S1004,S1012,S1019,S1048,S1049,S1053,S1063,S1070,S1075共9个引物进行反应。依据随机引物对不同品种水稻总DNA扩增出的特异性扩增条带,用Ne i’s公式计算30个水稻品种间的相似系数,并以UPMGA法进行聚类分析,其结果与品种系谱基本吻合,证明RAPD标记可应用于水稻的分类。The present study used RAPD markers to study the relation of 30 rice cultivars. In the course of PCR, 9 primers were selected from 80 primers to screen the samples. These 9 primers have polymorphism, they are S1004 , S1012, S1019 , S1048 , S1049 , S1053 , S1063 , S1070 , S1071. Based on the pair wise comparisons of amplification products the genetic distance was calculated using Nei genetic distance and a dendrogram was constructed using an unweighted pair group method with arithmetical averages（ UPGMA）. These 30 rice cultivars were divided into 2 groups. This result was identical with pedigree analysis and proved that RAPD markers could be used in classification of rice cultivars.

关 键 词：水稻 籼粳分类 随机扩增多态性DNA 

分 类 号：S511[农业科学—作物学]