毛细管离子分析法测定人发中钙和锌  

DETERMINATION OF CALCIUM AND ZINC IN HUMAN HAIR BY CAPILLARY ION ANALYSIS

作　　者：舒友琴[1] 童岩[1] 

机构地区：[1]郑州牧业工程高等专科学校,郑州450008

出　　处：《理化检验（化学分册）》2006年第9期717-718,720,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：适量人发试样经洗涤、冲洗，并于60℃干燥至恒重后称取1．000g于600℃灰化后用盐酸（1＋1）溶解残渣，溶液定容为100mL供CIA分析。在10mmol·L^-1咪唑、10mmol·L^-1。α-羟基乙酸、pH4．0的电泳介质中，两离子在10min内实现了分离。以间接紫外法检测（214nm），外标法定量。钙（Ⅱ）及锌（Ⅱ）的峰面积与其质量浓度之间在0．5～40mg·L^-1范围内呈线性关系，相关系数分别为0．9991和0．9989，检出限（S／N：3）为0．1和O．2mg·L^-1，回收率在97．9%～102．5％之间，相对标准偏差小于2．5％。Appropriate amount of human hair sample was cleaned, washed and dried at 60℃ to constant weight and 1. 000 g of the dried sample was taken and ashed at 600℃. The residue was taken up with HCI（ 1＋1 ）, and the solution was made up to 100 mL in a volumetric flask and ready for capillary ion analysis （C1A）. In an electrophoretic medium of pH 4, 0, containing 10 mmol . L^-1 imidazol and 10 mmol . L^-1 cchydroxyacetic acid, Ca^2＋ and Zn^2＋ ion were separated within 10 min, and determined by indirect UV detection at the wavelength of 214 nm Quantification was performed by the external standard method. Linearity between the peak area and concentration of Ca^2＋ and Zn^2＋ ion were obtained both in the ranges of 0. 5- 40 mg . L^-1 with correlation coefficients of 0, 999 1 and 0. 998 9 respectively. Detection limits （S/N=3） were found to be 0. 1 mg . L^-1 （for Ca^2＋ ） and 0.2 mg .L^-1 （for Zn^2＋ ）. RSD＇s （n=6） found for this method were less than 2. 5%, and recoveries were in the range of 97. 9%--102. 5%.

关 键 词：毛细管电泳 毛细管离子分析 间接紫外检测 人发 钙 锌 

分 类 号：O657.8[理学—分析化学]