海蒿子多糖DEI、DEII组分的分离纯化及单糖组成分析  

The studies on the isolation,purification,and chemical composition analysis of polysaccharides DEI and DEII from Sargassum confusum

作　　者：赵宇[1] 李俊卿[1] 张立新[1] 陈悦[1] 李志萍[1] 陆懋荪[1] 尹佩玉[1] 

机构地区：[1]青岛大学化工学院,山东青岛266071

出　　处：《海洋科学》2006年第9期6-8,共3页Marine Sciences

基　　金：青岛市科技局资助项目(02-1-kchhh-42-2)

摘　　要：海蒿子(SargassumconfusumAgardh)经热水抽提、乙醇沉淀、DEAE-SephadexA-25柱层析分离,得到多糖DEI、DEII,糖质量分数分别为52.40%,38.80%。经酸降解、薄层层析、气相色谱-质谱分析,证明其单糖组成均为岩藻糖、木糖、半乳糖、果糖、甘露糖、葡萄糖。其中岩藻糖质量分数最高(DEI:39.3%;DEII:35.2%)。The characteristics of the polysaccharide DEI and polysaccharide DEll isolated from Sargassum pallidium through water extraction, ethanol precipitation and DEAE-Sephadex A-25 column chromatography sequentially were analyzed by TLC, gel electrophoresis and GC-MS. It was found that these polysaccharides were composed of fucose, xylose, galactose, fructose, mannose and glucose, among them fucose being the primary component （39.3% in DEI and 35.2% in DEII）.

关 键 词：海蒿子(Sargassum comfusum Agardh) 多糖 分离 纯化 单糖组成 

分 类 号：R284.1[医药卫生—中药学]