转化生长因子β1对糖尿病骨代谢影响的实验研究  

The changes of TGFβ_1 in experimental diabetic osteoporosis and their clinical significances

作　　者：邢学农[1] 丁敏[2] 李素梅[1] 叶山东[1] 陈燕[1] 任安[1] 陈若平[1] 莫蔚林[1] 

机构地区：[1]安徽医科大学附属省立医院安徽省立医院内分泌科,合肥230001 [2]安徽医科大学附属省立医院安徽省立医院病理科,合肥230001

出　　处：《中国临床保健杂志》2006年第5期427-430,共4页Chinese Journal of Clinical Healthcare

摘　　要：目的了解转化生长因子(TGF)β1与糖尿病性骨质疏松症之间的关系,及血管紧张素转换酶抑制剂福辛普利和血管紧张素Ⅱ受体拮抗剂氯沙坦对骨质疏松症的治疗作用。方法2月龄雄性大鼠20只,随机分为糖尿病组(A组)、福辛普利治疗组(B组)、氯沙坦治疗组(C组),正常对照(D组)。检测各组第1周及第12周血糖、24 h尿TGFβ1排泄率;随后测定各组大鼠股骨BMD并镜下观察骨形态学改变情况。结果第1周尿TGFβ1排泄率A、B、C三组均高于D组(P<0.01);第12周尿TGFβ1排泄率前三组明显高于D组(P<0.01),B、C二组低于A组(P<0.01)。股骨骨密度(BMD)A、B、C三组均低于D组(P<0.01),但A、B、C三组间差异无统计学意义。镜下可见A组股骨小梁明显变细,稀疏且断裂,B、C二组骨小梁情况优于A组,与D组比较仍差。结论1型糖尿病可影响雄性大鼠正常骨代谢,导致骨疏松症;尿TGFβ1排泄率增加似乎与骨质疏松关系不大;ACEI/ATRA治疗可部分改善糖尿病雄性大鼠骨质疏松症。Objective To observe the relationship between TGFβ1 and diabetic osteoporosis in experimental rats, and to explore the effects of angiotensin converting enzyme inhibitor（ACEI）-fosinopril and angiotensin Ⅱ receptor antagonist（ ATRA）-losartan on osteoporosis. Method 20 male Wistar rats, two months old, were randomly dividied into 4 groups. Group A ： diabetic rats ; group B： diabetic rats treated with fosinopril; group C ： diabetic rats treated with losartan; group D： normal control rats. Blood glucose and urine TGFβ1, were measured at the 1st and 12th weeks. Then femur BMD of each rat were detected, and histological changes of bone were observed. Results At the 1 st week,the urinary excretion rates of TGFβ1 in groups A, B,C were higher than those in group D, at the 12th week, this parameter significant＇g increased in groups A ,B,C compared with those in group D （P 〈0.01 ）. The femur BMD were lower in groups A ,B ,C than those in group D,while no significant difference among groups A ,B ,C. The trabecular bone in group A were significant thin, sparse and broken; while they were better in groups B, C. Conclusions T1DM may induce osteoporosis in male rats by affecting bone metabolism ; The increased urinary excretion rates of TGFβ1 seems to be not related with osteoporosis. Treatment with ARB/ACEI might improve partly diabetic osteoporosis in male rats.

关 键 词：糖尿病并发症 转换生长因子β 骨质疏松 模型 动物 

分 类 号：R587.2[医药卫生—内分泌]