小麦D^2型细胞质雄性不育恢复基因近等基因系筛选和遗传背景的分子检测  

Screening of the Near-isogenic Lines (NILs) of Fertility Restorer Genes for D^2-type Cytoplasmic Male Sterility (CMS) in Wheat and Molecular Characterization of the Genetic Backgrounds

作　　者：刘立科[1] 侯宁[1] 刘建成[1] 刘根齐[1] 刘春光[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所植物细胞与染色体国家重点实验室,北京100101

出　　处：《麦类作物学报》2006年第5期1-4,15,共5页Journal of Triticeae Crops

基　　金：973项目(001CB1088);863项目(2002AA207004);中科院知识创新工程项目(KSCX2-1-01)

摘　　要：通过连续回交和单株(系)跟踪选择,培育成小麦D2型细胞质雄性不育系msD2-CA8057恢复基因的近等基因系(BC6F1)材料。应用SSR分子标记技术,对9个恢复基因近等基因系单株及不育系msD2-CA8057和恢复系遗4060的遗传背景进行了比较检测分析,结果表明,所检测到的遗传多样性集中于近等基因系与恢复系之间,而9个近等基因系单株之间及其与不育系之间的遗传背景具有很高的一致性;近等基因系C3只含一个主效恢复基因D2Rf1,该单株的自交和回交群体可望用于对该基因的精细定位。By successive backcrossing and individual selection, the near-isogenic lines （NILs） of fertility restorer genes for D^2-type cytoplasmic male sterility （CMS） in wheat were bred. Using microsatellite marker （SSR）, the genetic backgrounds of 9 NILs were obtained through compared to that of CMS line msD^2-CA8057 and restorer line Yi 4060, respectively. The results indicated that the polymorphisms mainly existed among the 9 NILs were highly distributed between the all 9 NILs and msD^2- CA8057. A NIL, C3, which contained one major restorer gene D^2Rf1, was obtained, and its selfcrossing and backcrossing populations could be used for fine mapping of D^2Rf1 gene.

关 键 词：小麦D^2型细胞质雄性不育系 近等基因系 恢复基因 SSR分子标记 

分 类 号：S512.1[农业科学—作物学] S334.5