ACGM标记在小麦属中的通用性  

Study on the Transferability of ACGM in Triticum

作　　者：卢泳全[1] 吴为人[1] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310029

出　　处：《麦类作物学报》2006年第5期16-19,共4页Journal of Triticeae Crops

基　　金：国家"863"计划项目(2003AA207160);福建省重大科技专项项目(2004NZ01-2)

摘　　要：为检验水稻基因组数据在小麦属中的通用性,利用基于水稻基因组数据设计的扩增共有序列遗传标记(ACGM)扩增小麦属12种不同材料,结果有32对引物可以至少在1种小麦属材料中获得扩增产物,占引物总数的80%;在能够获得特异性产物的32对引物中,有4对引物的产物在供试材料之间没有多态性位点;有28对引物可以在供试小麦材料之间获得多态产物,占引物总数的70%。这表明以水稻基因组序列开发的ACGM标记在小麦中有一定的通用性。In order to study the transferability of rice genome data, 40 ACGM markers exploited from rice were investigated in 12 Triticum accession in this study. A total of 32 pairs of primers exhibited specific PCR products in at least one accession, occupying 80% of total primers. Among those 32 pairs of primers exhibited specific PCR products, 4 have no polymorphism among accessions. A total of 28 pairs of primers exhibited polymorphis PCR products among all accessions, occupying 70% of total primers. This result shows that rice,genome data have some transferability in triticum.

关 键 词：小麦属 ACGM标记 扩增 

分 类 号：S512.1[农业科学—作物学] S326