黄淮麦区小麦品种高分子量谷蛋白亚基组成及遗传多样性分析  

Compositions of High Molecular Weight Glutenin Subunits (HMW-GS) and Their Genetic Diversity in Wheat Cultivars from Huang-Huai Wheat Region

作　　者：王银萍[1] 李勇超[1] 魏燕燕[1] 刘林丽[2] 赵惠贤[1] 郭蔼光[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]西北农林科技大学生命学院生物技术实验室,陕西杨凌712100

出　　处：《麦类作物学报》2006年第5期69-73,共5页Journal of Triticeae Crops

基　　金：国家转基因研究与产业化开发专项课题(JY03-A-11-01)

摘　　要：为给小麦育种和面粉的深加工提供参考依据，利用十二烷基硫酸钠一聚丙烯酰胺凝胶电泳（SDS-PAGE）方法对黄淮五省区50份主推小麦品种的高分子量谷蛋白亚基（HMW-GS）组成进行了研究，并按照Payne等的评分方法计算其Glu-1位点的品质得分。结果表明，黄淮麦区小麦品种中HMW-GS变异较为丰富，在参试材料中共检测到12种不同的亚基类型和17种HMW-GS组合类型，品质平均得分在5．5～7．5分之间。根据HMW-GS组成，可将其聚为3大类，第一大类包括7个品种，其Glu-1品质评分最高，为8～10分；第三大类有4个品种，该类品种品质评分很低，为5分；其它小麦品种被聚于第二大类，其品质评分变化幅度较大，为4～8分。The HMW-GS compositions of wheat cultivars from Huang-Huai region were analyzed by SDS-PAGE,the quality scores of each cultivar at Glu-1 were calculated according to the method described by Payne et al. The results showed that the variations of HMW-GS among wheat cultivars in this region were abundance. There were 12 kinds of allelic variations the tested cuhivars and 17 combinations of HMW-GS were found. The quality scores of Glu-1 of the tested cultivars in this study were between 5.5 and 7.5. The tested cultivars were classified into 3 groups based on their HMW-GS compositions. The group Ⅰ includes 7 cultivars and the range of their quality scores was 8～10. The Group Ⅲ includes 4 cultivars and their quality score was 5. The other cultivars were classified into the group Ⅱ and the range of their quality scores was 4～8. These results can provide very useful information for rational utilization of these wheat cultivars in wheat improvement .

关 键 词：小麦 高分子量谷蛋白亚基 遗传多样性 

分 类 号：S512.1[农业科学—作物学] S331