槐果碱对感染CVB3搏动心肌细胞的保护作用  

Protection of cardiomyocytes from coxsackievirus B3 by sophocarpine

作　　者：张星宇[1] 陈曙霞[2] 刘晶星[3] 钱富荣[2] 

机构地区：[1]上海交通大学医学院附属仁济医院急诊科,上海200127 [2]上海交通大学医学院附属仁济医院心内科,上海200127 [3]上海交通大学基础医学院微生物教研室,上海200025

出　　处：《中国新药与临床杂志》2006年第9期709-712,共4页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的：应用柯萨奇病毒B3(CVB3)感染SD大鼠乳鼠心肌细胞造成病毒性心肌炎模型,观察槐果碱对该模型的作用。方法：(1)将乳鼠心肌细胞分成4组：感染组,感染CVB3；治疗组,感染病毒后加入100mg·L^(-1)的槐果碱；同时设立药物对照组和细胞对照组。在处理的d 2,3,5观察各组细胞病变效应(CPE)、细胞搏动频率并测定细胞上清液中乳酸脱氢酶浓度。(2)将心肌细胞感染CVB3后加入不同浓度的槐果碱(12．5～400mg·L^(-1)),设立病毒、细胞及药物对照。在处理后d 2,3,5观察CPE并测定细胞上清中的乳酸脱氢酶(LDH)浓度。结果：(1)100mg·L^(-1)的槐果碱对感染CVB3的乳鼠心肌细胞有保护作用,治疗组的CPE较感染组减轻,上清液中的LDH浓度降低。(2)槐果碱在12．5～300mg·L^(-1)之间均对感染CVB3的乳鼠心肌细胞有保护作用,它可以减轻病毒导致的CPE,并减少LDH释放。在400mg·L^(-1)反而会加重CPE,增加LDH释放。结论：一定浓度的槐果碱在体外对感染CVB3的心肌细胞有保护作用。AIM： To observe the effect of sophocarpine （SC） on the myocarditis model established by coxsackievirus B3 （CVB3） in cardiomyocytes from neonatal rats. METHODS： Cardiomyocytes from neonatal rats were divided into four groups： infected group, which was infected by CVB3; SC treated group, which was incubated with SC （100 mg.L^-1） after viral infection; SC control group, which was incubated with SC （100 mg. L^-1） only; and cell control group. The cytopathic effect （CPE）, beating frequency of myocardial cells and the lactate dehydrogenase （LDH） levels in supernatant was tested at day 2, 3, 5 respectively after the treatment. The cultured myocytes were incubated with different concentrations of SC （ 12.5 - 400 mg.L^-1） after infected by CVB3. The CPE and the levels of LDH were tested at day 2, 3, 5 after treatment. RESULTS： （1） SC had protective effect on CVB3-infectcd cardiomyocytes. The cytopathic effect was lighter and the LDH level was lower in SC treated group （100 mg.L^-1） than that in infected group. （2） SC in concentration between 12.5 - 300 mg. L^-1 could protect the cardiomyocytes from CVB3; it improved CPE and decreased the LDH levels. While in a higher concentration （400 mg.L^-1） , SC exacerbated the CPE and increased LDH levels. CONCLUSION： SC in certain concentration could protect cardiomyocytes from CVB3 in vitro.

关 键 词：柯萨奇病毒感染 心病毒感染 细胞培养 槐果碱 

分 类 号：R373.23[医药卫生—病原生物学] R542.21[医药卫生—基础医学]