老年性雀斑样痣和皮肤老化的表皮-真皮黑素轴和噬黑素细胞因子XIIIa的改变  

Alterations in the epidermal-dermal melanin axis and factor XIIIa mel anophages in senile lentigo and ageing skin

作　　者：nver N. Freyschmidt-Paul P. Hrster S. H.-P. Elssser 朱国兴 

机构地区：[1]Institut fü r Zytobiologie und Zytopathologie, Philipps University of Marburg, Robert-Koch Str. 6, 35033 Marburg, Germany

出　　处：《世界核心医学期刊文摘（皮肤病学分册）》2006年第9期36-37,共2页Digest of the World Core Medical JOurnals:Dermatology

摘　　要：Background: Senile lentigo (SL) is a pigmentation disorder that occurs predominantly on the dorsa of the hands, the forearms and the face; its incidence increases with age. Histological hallmarks of SL lesions are hyperpigmentation of the epidermis and elongation of the epidermal rete ridges. Various factors such as α -melanocyte-stimulating hormone, endothelin-1 or stem cell factor are involved in the onset and maintenance of the increased pigmentation. Alterations of the dermal compartment have not yet been analysed in detail in SL. Objectives: To study the occurrence and distribution of melanin in the dermis from SL and aged skin, biopsies from 12 subjects were morphologically analysed by light and electron microscopy in comparison with unaffected skin. Methods: Punch biopsies of SL and adjacent skin from 12 male or female volunteers aged 52- 81 years were prepared for light and electron microscopy and samples were analysed by morphological, morphometric, histochemical and immunohistochemical methods. Results: The epidermis from SL revealed morphological features such as hyperpigmentation of basal keratinocytes and the formation of elongated rete ridges. S100+ melanocytes in the stratum basale were not markedly increased, indicating that the hyperpigmentation is predominantly due to changes in melanin synthesis, distribution or turnover. Quantification of epidermal cells expressing the proliferation marker Ki67 did not show an increase of this parameter in SL, indicating that at least in the established lesion cell proliferation is not enhanced. We further focused on the dermal compartment and observed granulated cells which were more abundant in SL. Electron microscopic and histochemical analysis revealed that the granulation of these cells is based on melanosomes, mostly present in large melanosomal complexes. Immunohistochemistry using antibodies to CD68 and factor XIIIa (FXIIIa) showed these melanophages to be predominantly FXIIIa+ dermal dendrocytes, which were about six times more abundant than CD68+ maBackground： Senile lentigo （SL） is a pigmentation disorder that occurs predominantly on the dorsa of the hands, the forearms and the face; its incidence increases with age. Histological hallmarks of SL lesions are hyperpigmentation of the epidermis and elongation of the epidermal rete ridges. Various factors such as α-melanocyte-stimulating hormone, endothelin-1 or stem cell factor are involved in the onset and maintenance of the increased pigmentation. Alterations of the dermal compartment have not yet been analysed in detail in SL. Objectives： To study the occurrence and distribution of melanin in the dermis from SL and aged skin, biopsies from 12 subjects were morphologically analysed by light and electron microscopy in comparison with unaffected skin. Methods： Punch biopsies of SL and adjacent skin from 12 male or female volunteers aged 52 - 81 years were prepared for light and electron microscopy and samples were analysed by morphological, morphomettic, histochemical and immunohistochemical methods. Results： The epidermis from SL revealed morphological features such as hyperpigmentation of basal keratinocytes and the formation of elongated rete ridges. S100＋ melanocytes in the stratum basale were not markedly increased, indicating that the hyperpigmentation is predominantly due to changes in melanin synthesis, distribution or turnover, Quantification of epidermal cells expressing the proliferation marker Ki67 did not show an increase of this parameter in SL, indicating that at least in the established lesion cell proliferation is not enhanced. We further focused on the dermal compartment and observed granulated cells which were more abundant in SL. Electron microscopic and histochemical analysis revealed that the granulation of these cells is based on melanosomes, mostly present in large melanosomal complexes. Immunohistochemistry using antibodies to CD68 and factor ⅩⅢa （FⅩⅢa） showed these melanophages to be predominantly FⅩⅢa ＋ dermal dendrocytes, which were about six times

关 键 词：Α-促黑素细胞激素 干细胞因子 皮肤老化 雀斑样痣 真皮层 老年性 表皮 色素沉着性疾病 

分 类 号：R619.6[医药卫生—外科学]