套式PCR用于恙虫病的早期诊断及恙螨幼虫体内立克次体的检测  

Detection of Rickettsia tsutsugamushi in Chigger Mites and Patients'blood Samples by Nested Polymerase Chain Reaction

作　　者：严延生[1] 王惠榕[1] 郑兆双[1] 何似[1] 于恩庶[1] 廖灏溶[1] 李世清[1] 何培瑞 俞云彬 李榕晃 沈桂成 

机构地区：[1]福建省卫生防疫站,福清市卫生防疫站,福清三山镇卫生院

出　　处：《中国人兽共患病杂志》1996年第5期12-15,共4页Chinese Journal of Zoonoses

摘　　要：从恙虫病立克次体（Ｒｔ）Ｋａｒｐ株的群特异性抗原基因序列设计两对引物建立套式ＰＣＲ检测现场鼠体采集的恙螨幼虫体内及恙虫病现症病人外周血单核细胞内的ＲｔＤＮＡ。除一份已用过药病人标本外，其余９份均可检见８８ｂｐＤＮＡ扩增产物；检测结果表明：现场鼠体采集的地里纤恙螨（Ｌｅｐｔｏｔｒｏｍｂｉｄｉｕｍｄｅｌｉｅｎｓｅ）幼虫的Ｒｔ携带率是１３．３％，证实套式ＰＣＲ可用于急性期恙虫病的诊断和媒介恙螨流行病学的调查。A nested PCR method was developed by using the primer pairs designed from 58-kDa antigen gene of Rickettsia tsutsugamushi Karp strain. DNAs extracted from peripheral blood mononuclear cells (PBMC) from patients with tsutsugamushi disease who were seen 3～9 days after the disease onset and from chigger mites collected from rodents in an epidemic area, were detected by the test. Except one that was obtained from a patient who had accepted drug treatment two days before the test, nine PBMC samples from the patients showed positive for rickettsial DNA while only four patients were serodiagnosed by immunofluorescent assay. The detection of the larvae from Leptotrombidium dilience indicated that 13.3% of them carried R. tsutsugamushi. The results showed that the PCR assay could be a useful tool for diagnosis of acute tsutsugamushi disease and epidemic investigation.

关 键 词：恙虫病 立克次体 地里纤恙螨 诊断 

分 类 号：R513.104[医药卫生—内科学]