废水中2,6-二硝基酚厌氧毒性和降解动力学研究  

Study on anaerobic toxicity and degradation kinetics of 2,6-DNP

作　　者：吴美玲[1] 佘宗莲[1] 汤改风[1] 

机构地区：[1]中国海洋大学环境科学与工程学院海洋环境与生态教育部重点实验室,青岛266003

出　　处：《环境污染治理技术与设备》2006年第9期31-34,共4页Techniques and Equipment for Environmental Pollution Control

基　　金：国家自然科学基金资助项目(40272108)

摘　　要：在中温（35℃±1℃）厌氧条件下，以葡萄糖为共基质，采用间歇实验方法，研究了2，6-二硝基酚（2，6-DNP）的厌氧产甲烷毒性和厌氧降解动力学。厌氧毒性试验（ATA）以累计产甲烷量和相对活性（RA）为指标，评价了不同浓度2，6-DNP对产甲烷菌的抑制程度；结果表明，2，6-DNP浓度＜20mg／L时，对产甲烷菌没有抑制作用，浓度为40mg／L时产生轻度抑制，浓度为80～120mg／L时产生重度抑制；24h2，6-DNP的75％、50％、25％相对抑制浓度分别为30、70和＞120mg／L。2，6-DNP降解动力学可用Haldane方程来描述，利用非线性拟合求得动力学参数Ks、Rm、Ki分别为179．7mg／L、4．84mg／gVSS·h、206．5mg／L，方差R^2=0．94，拟合效果很好。Anaerobic toxicity assay （ATA） and degradation kinetics of 2,6-dinitrophenol （2,6-DNP） were investigated in batch culture. The tests were conducted at the temperature of 35℃±1℃ and glucose were used as co-substrate. In anaerobic toxicity assay, accumulative methane production and relative activity （RA） were used to judge the inhibition level of 2,6-DNP on methanogenic bacteria. The results indicated that 2,6-DNP had no inhibition effect on the activity of methanogenic bacteria when the 2,6-DNP concentration was below 20 mg/ L, 2,6-DNP caused slightly inhibition when its concentration was 40 mg/L, and strong inhibition when its concentration was from 80 mg/L to 120 mg/L. The 75% , 50% and 25% relative inhibition concentrations of 2,6- DNP for 24-hour reaction was 30 mg/L, 70 mg/L and 〉 120 mg/L respectively. Haldane equation was proposed to describe the degradation of 2,6-DNP, and the kinetic model parameter Ks, Rm and Ki were estimated by nonlinear curve fit to be 179.7 mg/L, 4.84 mg/ g VSS·h and 206.5 mg/L respectively. The experimental data verification for the model equation is satisfactory. R^2 = 0.94.

关 键 词：2 6-二硝基酚 葡萄糖 厌氧毒性 厌氧降解动力学 

分 类 号：X703.1[环境科学与工程—环境工程]