识别血吸虫成虫多肽表位单克隆抗体细胞株的建立及用于检测循环抗原的研究  

Establishment of McAb Specifically Recognized Polypiptide Epitopes for Detecting Circulating Antigen of Schistosoma japonicum

作　　者：黄启华[1] 吴子松[1] 高鹏 张桂威 邱东川[1] 周国兴[1] 山建忠 蔡兴平[1] 

机构地区：[1]四川省寄生虫病防治研究所,四川省眉山县血防站

出　　处：《中国人兽共患病杂志》1996年第5期38-39,33,共3页Chinese Journal of Zoonoses

基　　金：UNDP/WorldBack/WHO/TDK资助

摘　　要：作者获得１株特异的抗血吸虫成虫２０－２４ｋＤａ蛋白多肽表位的杂交瘤细胞株（ＳｊＡ１１１）。分泌ＭｃＡｂ为ＩｇＧ１，腹水效价１．２×１０６。将ＳｊＡ１１１ＭｃＡｂ－ＨＲＰ结合物用Ｄｏｔ－ＥＬＩＳＡ检测４９９例慢性血吸虫病患者的循环抗原（ＣＡｇ），阳性者３９０例（７８．１６％）；２５例肺吸虫病人，３６例华支睾吸虫病人及１３２例正常人血清中，仅１例肺吸虫病人出现交叉反应。ＣＡｇ的检出率和阳性血清滴度倒数的几何均值（ＧＭＲＴ）随着每ｇ粪便虫卵数（ＥＰＧ）的增加而递增。吡喹酮治疗后各期病人粪孵阴性者ＣＡｇ的转阴率：半年为７５．５０％（１１４／５１），１年为７３．６８％（８４／１１４），１年半为８８．０４％（１０３／１１７）。A hybridoma cell line (Sj A111) secreting McAb of 20-24kDa polypiptide epitopes from adult worm of S. japonicum has been prepared. The immunoglobulin class of SjA111 McAb was IgG1. The titer of ascited containing McAb reached 1.2×106. 499 cases with chronic schistosomiasis were detected by Dot-ELISA using SjA111 McAb-HRP conjugate. Positive rate of CAg was 78.15% (390/499). Only 1 out 25 cases with paragonimiasis showed the cross reaction while other 36 cases with clonorchiasis and 132 normal individuals showed negative reaction. The detection rate and GRMT of CAg corresponded with the increase of EPG. Six months, 1 year and 1. 5 years after effective treatment. The negative conversion rate of CAg became 75.5% (114/151), 73.69% (84/114) and 88. 0% (103/117) respectively.

关 键 词：血吸虫 多肽表位 ELISA 单克隆抗体 细胞株 

分 类 号：R383.24[医药卫生—医学寄生虫学]