桉树组培工厂化育苗中培养基调配技术研究  

Adjustment Technique of Culture Medium in Industrialization of Seedling production of Eucalyptus

作　　者：王志洁[1] 

机构地区：[1]福建省林业科学研究院,福建福州350012

出　　处：《福建林业科技》2006年第3期85-87,100,共4页Journal of Fujian Forestry Science and Technology

基　　金：福建省林业厅种苗重大攻关项目"桉树良种选育与扩繁技术应用研究"的部分内容

摘　　要：对桉树组培工厂化育苗培养基的调配进行了系统的研究，以尾巨桉DH32—29优良无性系组培苗为试验材料，探讨了桉树组培工厂化育苗生产中影响的关键因素，结果表明：琼脂成分、激素浓度和种类、大量元素含量对组培苗生长有显著影响。继代培养阶段，培养基中添加琼脂条时变异苗率低且平稳，增殖阶段，培养基中激素6-BA和NAA的浓度分别在0．25～0．30mg·L^-1和0．10～0．15mg·L^-1范围内时增殖效率最好。壮苗阶段，培养基中激素6-BA和NAA的浓度分别在0．25～0．27mg·L^-1和0．10～0．15mg·L^-1范围内时有效出苗率最佳。生根培养阶段，培养基中添加浓度为40％的大量元素。其生根率达95％，且不影响后续生长，生根激素在继代培养3代以后可降低为ABT0．5mg·L^-1和NAA为0．5mg·L^-1。Take the tissue culture seedlings of Eucalyptus urophyUa× E. grandis DH32-29 clone as experiment material, adjustment technique of culture medium and key factors were studied in industrialization of seedling production of eucalyptus in the paper. The result showed that, the composition of agar, the concentration and kinds of hormone, and the contents of macro-element had distinct influence on the growth of tissue culture seedlings. At the subculture phase, the rate of variation seedlings was very low and stable when agar bars was added in the culture medium; at the proliferative phase, the highest proliferative efficiency was gained when 6-BA （0.25-0.30 mg·L^-1） and NAA （0.10--0.15 mg·L^-1） was added in the culture medium; at the strength seedling phase, the highest tissue culture seedling yield was produced as added 6-BA （0.25- 0.27 mg· L^- 1） and NAA （0.10- 0.15 mg· L^ - 1 ） to the culture medium; And at the rhizogenic phases, the rate of rhizogenesis reached to 95 % when adding macroelement with concentration of 40 %, and that had no any influence to the future growth. After third generation of subculture, the concentration of ABT and NAA could be decreased to 0.5 mg· L^- 1 and 0.5 mg· L^- 1 respectively for best rhizogenesis.

关 键 词：桉树 工厂化育苗 培养基调配技术 

分 类 号：S792.390.5[农业科学—林木遗传育种]