C-erbB-2特异性siRNA逆转录病毒载体的构建与鉴定  

Construction and Identification of Recombinant Retroviral Vector of siRNA Specific for C-erbB-2

作　　者：陈敏[1] 祝怀平[1] 凌斌[1] 周颖[1] 朱园园[1] 高婷[1] 程志祥[1] 沈国栋[1] 赵卫东[1] 冯定庆[1] 

机构地区：[1]安徽省分子医学重点实验室,安徽医科大学附属安徽省立医院,合肥230001

出　　处：《临床输血与检验》2006年第3期161-164,共4页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：安徽省自然科学基金(No.20050430715);安徽省教育厅科研基金(No.2005KJ347ZC)资助

摘　　要：目的构建并鉴定C-erbB-2特异性siRNA逆转录病毒载体。方法体外合成含针对C-erbB-2特异基因序列的寡核苷酸,并定向克隆入逆转录病毒载体RNAi-ReadypSIREN-RetroQ。结果构建的重组逆转录病毒载体可以被限制性内切酶MIuI、BamHI、EcoRI切开,电泳可显示相应条带,经测序其序列与设计的一致。结论成功构建了C-erbB-2特异性siRNA逆转录病毒载体pRetroQ/C-erbB-2/siRNA-1、pRetroQ/C-erbB-2/siRNA-2、pSIREN-RetroQ/C-erbB-2/siR-NA-3,为进一步研究p185基因沉默奠定了基础。Objective To construct and identify recombinant retroviral vectors of siRNA specific for C-erbB-2. Methods We synthesized oligonucleotides for C-erbB-2 in vitro and cloned them into retroviral vector RNAi-Ready pSIREN-RetroQ. Results The recombinant retroviral vectors digested by MIu I,BamH I,EcoR I showed the corresponding bands, and their sequences were the same to the anticipation. Conclusion The recombinant retroviral vectors of pSIREN-RetroQ/C-erbB-2/siRNA-I,pSIREN-RetroQ/C-erbB-2/siRNA-2 and pSIREN-RetroQ/C-erbB-2/siRNA-3 specific for C-erbB-2 were constructed successfully,which contributed to the study of p185 gene silencing.

关 键 词：C-ERBB-2 RNA干扰 逆转录病毒载体 

分 类 号：R392.11[医药卫生—免疫学] R73[医药卫生—基础医学]