NFκB在白血病细胞株中持续激活状态的检测及其意义  

The Activation of NFκB in Leukemia Cells and its Significance

作　　者：周颖[1] 程勇[2] 沈国栋[1] 赵卫东[3] 朱园园[1] 陈敏[1] 冯定庆[1] 凌斌[1] 汪思应[4] 

机构地区：[1]安徽省分子医学重点实验室,合肥230001 [2]安徽省立医院肿瘤放疗科 [3]安徽省立医院妇产科 [4]安徽医科大学病理生理学教研室

出　　处：《临床输血与检验》2006年第4期260-261,共2页Journal of Clinical Transfusion and Laboratory Medicine

基　　金：国家自然科学基金(No.30170464)资助

摘　　要：目的 检测NFκB在白血病细胞株中的激活状态。方法 提取15种白血病细胞株的细胞核蛋白，与γ-^32p标记NFxB寡核苷酸共浴后垂直电泳，4～8h后放射自显影。结果 NFκB在15种白血病细胞株中均处于激活状态。结论 NFκB的激活可能与白血病的发病机制有关。Objective To explore the activation of NFκB in 15 kinds of leukemia ceils. Methods The nuclear protein of 15 kinds of leukemia cells were isolated. After bathing with NFκB oligonucleotide labeled with γ-^32p in room temperature,the nuclear protein was detected by vertical electrophoresis ,and the results were shown by exposure to X-ray film after 4～8 h. Results The NFκB DNA binding activity was enhanced in all kinds of leukemia cells. Conclusions The activation of NFκB in leukemia cells may play a role in the mechanisms of leukemia.

关 键 词：白血病 NFΚB 

分 类 号：R392.11[医药卫生—免疫学] R733.7[医药卫生—基础医学]