致敏患者的PRA水平对肾移植术后排斥反应的影响  

The effect of PRA level in pre-immuned recipient on early rejection after kidney transplantation

作　　者：那万里[1] 杨绍娟[2] 郭宝富[3] 傅耀文[1] 

机构地区：[1]吉林大学中日联谊医院泌尿外科,吉林长春130033 [2]吉林大学中日联谊医院中心研究室,吉林长春130033 [3]吉林大学中日联谊医院麻醉科,吉林长春130033

出　　处：《中国实验诊断学》2006年第8期907-908,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨致敏肾移植患者术前群体反应扩体(PRA)水平与移植后早期排斥反应的关系。方法采用酶联免疫吸附分析法(ELISA)对321例患者检测肾移植术前PRA;单克隆抗体干板进行供受者HLAI类抗原分型;微量序列特异性引物(Microssp)进行HLAⅡ类基因分型。结果PRA阴性组的早期排斥反应发生率明显低于致敏组;配型组的早期排斥反应发生率明显低于未配型组。结论PRA是影响移植后发生早期排斥反应的重要因素,降低PRA水平,并选择良好的配型供肾,能降低排斥反应的发生。Objective To study the relationship between the population reactive antibody（PRA） level in pre-immuned recipients before kidney transplantation and early rejection aftrer transplantation. Methods The PRA level of 321 patients were tested by ELISA;donor and recipient HLA class Ⅰ typing was performed with monoclonal antibody way and HIA class Ⅱ gene typing with Microsequence-specific-primevs（Micro-SSP）. Results The incidence of early rejection in the PRA negative group was much lower than that of pre-immuned group,and so was with the HLA matching group lower than HLA un-matching group. Conclusion PRA play an important role in early rejection after kidney transplantation. It will be possible to reduce early rejection by lowering the level of PRA and selecting suitable HLA matching donor.

关 键 词：肾移植 群体反应性抗体 HLA 早期排斥反应 

分 类 号：R699.2[医药卫生—泌尿科学]