人胎肝中两种抑瘤活性成分的分离和结构鉴定  

Isolation and structure identification of two constituents with antitumor activity from human fetal liver

作　　者：张庆林[1] 吴祖泽[1] 曹菊荣[1] 薛惠华[1] 冯锐[1] 杜泽涵[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《军事医学科学院院刊》1996年第4期266-268,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金

摘　　要：胎肝组织中存在低分子肿瘤抑制物。胎肝细胞悬液或裂解液用甲醇－丙酮提取后保留有大部分抑瘤活性。体外液体培养条件下以其对人急性粒系白血病细胞系（ＨＬ－６０）的抑制作用为指标，跟踪活性成分的分离过程。提取物经反相Ｃ１８中压液相色谱、ＳｅｐｈａｄｅｘＬＨ－２０凝胶过滤色谱及正相高压液相色谱分离得到两种活性物质，经核磁共振和高分辨率质谱仪鉴定为７－酮基胆固醇（７－ｋｅｔｏｃｈｏｌｅｓｔｅｒｏｌ，７－ＫＣ）和７β－羟基胆固醇（７β－ｈｙｄｒｏｘｙｃｈｏｌｅｓｔｅｒｏｌ，７β－ＨＣ）。In this paper, two suppressors were separated and purified from methanol extract of human fetal liver, with the isolation process directed by suppression of HL 60 cells growth in vitro. The procedure for purification included C18 reversed phase medium pressure chromatography, gel filtration on Sephadex LH 20, and high performance liquid chromatography. The structures of the suppressors were identified to be 7 ketocholesterol and 7β hydroxycholesterol by high resolution mass spectrometer and nuclear magnetic resonance.

关 键 词：肝 胎儿肝 低分子抑瘤物 分离 结构 鉴定 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学] R73-34[医药卫生—基础医学]