枯草芽孢杆菌Bs-1拮抗溶藻弧菌的特性  

Properties of metabolites from marine-derived Bacillus subtilis antagonistic to Vibrio alginolyticus

作　　者：黄汝添[1] 谢海平[2] 陆勇军[1] 吕军仪[1] 吴金英[1] 

机构地区：[1]中山大学生命科学院,广东广州510275 [2]广东省海洋与渔业环境监测中心,广东广州510222

出　　处：《热带海洋学报》2006年第4期51-55,共5页Journal of Tropical Oceanography

基　　金：国家"863"高技术研究发展计划(2002AA624010)项目;广东省自然科学基金项目(031623)

摘　　要：研究了枯草芽孢杆菌Bacillus subtilis(Bs-1)抗菌物质产生的特点及培养液的无菌滤液的抗菌谱和理化性质,结果表明Bs-1生长快速,在5h内产生抗菌物质;该物质能抑制多种分离自养殖大海马Odobenus rosmarus肠道和海水的弧菌如溶藻弧菌Vibrio alginolyticus的生长;在一定范围内该物质对热、酸碱、变性剂和蛋白酶均有较好的稳定性。进一步尝试用Bs-1的含菌培养液来控制养殖海水中弧菌的数量,结果表明每升海水中加入4ml培养液(浓度为4×106CFU.ml?1)已能达到较好的抑菌效果;改变海水的pH和盐度,Bs-1培养液仍有较理想的抑菌作用。因此,Bs-1有望开发成良好的益生菌剂。Marine-derived Bacillus subtilis （Bs-1） grown in PDB medium produces a antimicrobial substance which can inhibit the growth of several marine bacteria isolated from sea horse＇s gut and sea water, like Vibrio alginolyticus, a fish pathogen. The production characteristics of the antimicrobial substance and the antimicrobial activity and spectrum of the cell-free cultural supernatant were studied. The results showed that Bs-1 grew rapidly and produced the antimicrobial substance in 5 hours. The results also showed that the substance was stable to heat, pH, denature reagent and proteinase to some extent. Further use of the culture supernatant of Bs-1 to control the Vibrio population demonstrated that the best result could be obtained when 4ml supernatant per liter was added to the water. The inhibiting activity was still kept at a relatively high level even if the pH value and salinity of the water were changed. Thus, Bs- 1 could probably be developed to be an efficient probiotic agent.

关 键 词：枯草芽孢杆菌Bacillus SUBTILIS 溶藻弧菌Vibrio ALGINOLYTICUS 海马Odobenus vosytlavus 益生菌 

分 类 号：Q935[生物学—微生物学]