低温海水腹腔浸泡对大鼠病理生理变化的影响  

Effect of Celiac immersion by Hepothermal Seawater on Pathophysiological Changes in Rats

作　　者：王志伟[1] 黄毅然[1] 吴印爱[1] 刘献棠[1] 罗真[1] 喻洪涛[1] 金浩生[1] 

机构地区：[1]解放军157医院普通外科,广州510510

出　　处：《华南国防医学杂志》2006年第4期19-21,共3页Military Medical Journal of South China

基　　金：广州军区卫勤类重点科研课题(2001A15)

摘　　要：目的观察低温海水腹腔浸泡对大鼠病理生理变化的影响。方法SD大鼠24只,随机均分为32℃海水浸泡组(A组)、22℃海水浸泡组(B组)及12℃海水浸泡组(C组),观察每一组腹腔海水浸泡前后平均动脉压(MAP)、心率(HR)、乳酸(LA)、乳酸脱氢酶(LDH)、谷丙转氨酶(ALT)、血淀粉酶(AMY)、尿素氮(BUN)及肌酸激酶同丁酶(CKMB)的变化。另取大鼠30只,随机均分为32℃海水浸泡组(D组)、22℃海水浸泡组(E组)及12℃海水浸泡组(F组),用以观察持续腹腔海水浸泡的存活时间。结果(1)F组平均存活时间仅为65分,显著少于D组(681分)及E组(287分)(P<0.01);(2)浸泡30分及浸泡后3小时B、C组MAP、HR与浸泡前及对照组比较均发生显著下降(P<0.05),而C组水平显著低于B组(P<0.05);(3)浸泡后3小时C组血浆LA、LDH、ALT、AMY、BUN及CKMB水平均显著高于A、B组(P<0.01)。结论低温海水浸泡腹腔可引起机体血流动力学迅速发生严重紊乱、代谢恶化及内脏功能损伤,引起大鼠快速死亡。Objective To observe the Effect of celiac immersion by hepothermal seawater on pathophysical changes in rats. Methods Twenty-four SD rats were randomly divided into three groups, i. e. 32℃ seawater immersion group（group A）, 22℃ seawater immersion group（group B）and 12℃ seawater immersion group （group C）. The changes in mean artery pressure （MAP） , heart rate（ HR） were ob served,and the plasma levels of lactic acid （LA）, LDH, ALT, AMY, BUN and CKMB were determined. Other thirty rats were randomly divided into 32℃ seawater immersion group（group D）, 22℃ seawater immersion group （group E） and 12℃ seawater immersion group （group F）,and the survival time during celiac seawater immersion was observed in every group. Results （1）The average Survival dine in group F was only 65min±12min, which was significantly lower C（ P 〈0.01 ） than that in groups D（681 min± 174min） and E（287 nm ± 5min）. （2） The MAP and HR in group B and C decreased significantly immediately and 3h after the celiac immersion compared with the preimmersion and group A, （ P 〈0.05 ） ,and MAP and HR in group C were significantly lower than that in group B（ P 〈0.05）. （3） The plasma levels of LA,LDH, ALT, AMY,BUN and CKMB in group C were significantly increased 3h after the celiac immersion compared with group A and B（ P 〈20.01）. Conclusion The celiac immersion by hepothermal seawater can produce severe changes in the hemodynamics and metabolism,and disfuncdon of multiple organs in the rats, thus rats die quickly.

关 键 词：腹腔 海水浸泡 低温 病理生理 

分 类 号：R826.65[医药卫生—临床医学]