粒细胞集落刺激因子对急性高原MODS心肌保护作用的研究  

Treatment on myocardium in multiple organ dysfunction syndrome rat at high altitude through Granulocyte Colony Stimulating Factor

作　　者：张勇[1] 梁家立[1] 姜冠华[1] 王惠[1] 郑晓舟[1] 杨哲[1] 张波[1] 

机构地区：[1]济南军区总医院,山东济南250031

出　　处：《山东医药》2006年第26期11-13,共3页Shandong Medical Journal

基　　金：全军医药卫生"十一五"科研项目(06MA102)

摘　　要：目的探讨粒细胞集落刺激因子(G-CSF)对高原急性多脏器功能不全综合征(M ODS)大鼠心肌的保护作用。方法在马汉山梯度(海拔3 900 m)建立20只大鼠M ODS模型,观察组(10只)造模前皮下注射G-CSF 10μg/(kg.d),连用5 d;对照组不用药。造模后24 h处死两组大鼠,取心肌标本,测定心肌梗死面积、左心室收缩及舒张期功能、心肌线粒体三磷酸腺苷(ATP)含量及F as mRNA、caspase-3的表达。结果观察组较对照组心肌收缩及舒张期功能增高明显,心肌ATP含量增加,心肌细胞凋亡数量减少,心肌中F as mRNA、caspase-3表达下降,心肌梗死面积缩小。结论G-CSF可提高高海拔地区M ODS大鼠心功能,其机制可能为动员骨髓基质干细胞修复坏死的心肌组织。[Objective]To study treatment on myocardium in multiple organ dysfunction syndrome rat at high altitude through Granulocyte Colony Stimulating Factor （G-CSF） . [Methods]The two-hit model of MODS rat was used at high altitude （3900m）. Before set up the model, G-CSF was administrated by Subcutaneous injection. Hemorrhage was induced in Wistar rat by catheterizing the femoral artery and bleeding until a mean arterial pressure = 35mmHg was reached and maintained for 1 hour. Rususcitation was performed was with lactated Ringer？ s. Cecal ligation and puncture（CLP） was performed 24 hours after hemorrhage. Rat was killed. Isolated hearts from male Wista rat weremounted on a Longendorff apparatus to estimate left ventricular systolic and diastolic function at different time points. The expression of Fas mRNA and the activity of Caspase-3 in myocardium was detected. Frozen myocardium was analyzed concentration of adenosine triphosphate. Myocardial infarct size was measured from mid-transverse sections stained with Masson＇s trichrome. [Results]Compared without G-CSF, the G-CSF can improved the left ventrlcular function and the concentration of myocardium adenosine triphosphate at high altitude more significant （P＇~0. 05）, the expression of Fas and Caspase-3 in myocardium at high altitude was lower （P〈0.05） and the myocardial infarct size was reduced. [Conclusion] Through applying the G-CSF, the marrow stromal cells moved and repaired the myocardium in MODS rat at high altitude. Our finding indicate that infusion G-CSF may represent a promising therapeutic strategy on myocardium of MODS rat at high altitude.

关 键 词：高原 多脏器功能不全综合征 心肌 粒细胞集落刺激因子 骨髓基质干细胞 

分 类 号：R654.1[医药卫生—外科学]