^(99)Tc^m-angiostatin的体内分布及其与新生血管作用的实验研究  

Study on the whole body distribution of ^(99)Tc^m-angiostatin and its effect on novel blood vessel

作　　者：陶永辉[1] 张莲芬[1] 尤徐阳[2] 万卫星[2] 金坚[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214036 [2]苏州大学附属第四医院,无锡214036

出　　处：《核技术》2006年第9期690-693,共4页Nuclear Techniques

基　　金：江苏省自然科学基金项目(BK2002071)

摘　　要：本文研究血管抑素(Angiostatin,AS)对血管内皮细胞作用的特异性。采用SnCl2还原法制备99Tcm-rhAS,通过鼠尾静脉注射99Tcm-rhAS,观察其体内分布并在小鼠S180肿瘤和Matrigel模型上进行显像及放射自显影,研究AS对新生血管的作用特异性。结果显示,99Tcm-rhAS在体内通过肝、肾进行代谢;S180肿瘤和Matrigel均可特异性摄取99Tcm-rhAS。S180肿瘤及Matrigel模型对99Tcm-rhAS特异性摄取表明AS可与新生血管特异性结合,其抑制肿瘤的作用是基于靶向血管内皮细胞的。The specificity of action of angiostatin on vascular endothelial cell is studied.^99Tc^m-angiostatin was prepared by the SnC12 reduced approach, then injected into the mice bearing S 180 tumor or Matrigel via tail vein. The whole body distribution and the effect of ^99Tc^m-angiostatin in vivo were observed on the model of mice bearing S 180 tumor and Matrigel by imaging and radioautography. The resultes indicate that the metabolism of ^99Tc^m-angiostatin is realized mainly by the liver and kidney pathway, and both S180 tumor and Matrigel can specifically uptake ^99Tc^m-angiostatin. The angiostatin＇s role of inhibiting tumor is found on the basis of targeting novel vascular endothelial cell.

关 键 词：血管抑素 显像 S180肿瘤 Matrigel模型 ^99TC^M 

分 类 号：R817.9[医药卫生—影像医学与核医学]