福建省12个地方解放钟枇杷的RAPD分析  

RAPD analysis for Jiefangzhong loquat from 12 sites of Fujian Province

作　　者：陈菁瑛[1] 陈义挺[1] 赖钟雄[2] 蔡英卿[2] 

机构地区：[1]福建省农科院果树研究所,福建福州350013 [2]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《亚热带农业研究》2006年第2期142-145,共4页Subtropical Agriculture Research

基　　金：福建省自然科学基金(B0310023);福建省科技厅重大资助项目(2004NZ02-2)

摘　　要：采用Sangon 13个长度为10 bp的引物对福建省12个地方的解放钟枇杷进行了RAPD分析,在12个样品中共扩增出64条带,不同引物获得的扩增带1-8条,S60最少,只有1条;S80最多,有8条;平均4.85条。其中11条引物对12个地方解放钟枇杷RAPD扩增结果都相同,而引物S66、S80对12个不同地方的解放钟枇杷进行RAPD扩增,结果表明3号在850bp、9号在250 bp处缺失1条扩增带,其余62条带都相同。这在DNA水平上证实了解放钟枇杷在遗传上保持较高的稳定性,但随着时间、环境的变化也会产生一些基因变异。Twelve accessions of Jiefangzhong loquat materials from 12 sites were investigated by RAPD analysis. 13 selected Sangon 10 bp primers generated 64 bands, in which amplified band number by different primers ranged from 1 （S60） to 8 （S80）, average band number amplified by each primer was 4.85. Genomic DNAs from 12 sites amplified by 11 primers generated same DNA bands, but genomic DNAs of the sample 3 amplified by S66 deleted one DNA band at 850 bp and sample 9 by S80 deleted one DNA band at 250 bp. The results indicated that genetic stability was high in Jiefangzhong, but some genetic variations occurred along with the changes of environments and time.

关 键 词：解放钟枇杷 RAPD 遗传变异 

分 类 号：S667.3[农业科学—果树学]