调肝颗粒剂抗免疫性肝损伤的实验研究  

Protective Effects of Tiaogan Granules on Immunologic Liver Injury in Mice

作　　者：陈忻[1] 赵晖[1] 张楠[1] 穆阳[1] 张秋云[1] 

机构地区：[1]首都医科大学中医药学院,北京100013

出　　处：《时珍国医国药》2006年第9期1680-1681,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的观察调肝颗粒剂对卡介苗(BCG)和脂多糖(LPS)所致小鼠免疫性肝损伤模型的保护作用,并探讨其作用机制。方法BCG(1×104个活菌)静脉注射致敏小鼠,12 d后静脉给LPS(6μg/只)攻击造成小鼠免疫性肝损伤,4 h后取血和肝脏,测血清AST和ALT并观察肝脏病理变化;乙酰氨基酚腹腔注射(500 mg/kg)致肝损伤,2.5 h后处死小鼠测血清GSH和肝脏MDA。结果给BCG的同时给调肝颗粒剂(20 g/kg)能显著的减轻BCG和LPS所造成的免疫性肝损伤小鼠ALT,AST的升高,减轻肝细胞坏死,促进枯否氏细胞增生,促进肝细胞增生;并能显著减轻对乙酰氨基酚致肝损伤引起的GSH降低,同时减少肝脂质过氧化物生成。结论调肝颗粒剂对BCG加LPS致小鼠免疫性肝损伤有显著的保护作用,这可能与它增加机体的抗氧化能力有关。Objective To investigate the protective effects of Tiaogan Granules on immunologic liver injury model. Methods （ 1 ） The liver injury model was induced by LPS intravenous injection in 12 days BCG interval, the mice were executed after 4h. the content of ALT and AST in serum were measured. （2） The other model was induced by paracetand ip 500 mg/kg, the mice were executed after 2.5h, the content of GSH and MDA in serum were measured. Results Tiaogan granules were affused （dose20g/kg） for the mice when BCG were injected by intravenous at the same time. The increase of ALT and AST were reduced by intervention of Tiaogan granules and putrescence of hepatic cell were reduced too, but hepatic were hyperplasia and hepatic cells were improved. The decrease of GSH and the increase of MDA on the mice injected paracetamol were reduced by intervention of the Tiaogan granules. Conclusion The protective effects of Tiaogan granules were confirmed on immunologic liver injury model. It was likely to be related to the enhancement of capability on resisted stress of oxidation of it.

关 键 词：中药 中药药理 免疫性肝损伤 保肝 

分 类 号：R285.5[医药卫生—中药学]