土鳖虫提取液对人胃低分化腺癌细胞BGC-823的抑制作用  

The Suppression Effect of Extract of Eupolyphaga on Human Gastric Low-differentia-tion Adenocarcinoma Cell Line BGC-823 in vitro

作　　者：邹玺[1] 刘宝瑞[1] 钱晓萍[1] 禹立霞[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肿瘤中心,江苏南京210008

出　　处：《时珍国医国药》2006年第9期1695-1696,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的研究土鳖虫体外对人胃低分化腺癌BGC-823细胞抗肿瘤活性。方法采用MTT法测定了土鳖虫水提物和醇提物对人胃低分化腺癌BGC-823细胞增殖的抑制作用,采用生长曲线测定法检测土鳖虫醇提物对体外培养肿瘤细胞的细胞毒作用。结果土鳖虫水提物不能抑制体外培养的肿瘤细胞,而醇提物能显著抑制人胃低分化腺癌BGC-823细胞增殖,药物作用48h时抑制率最高,并有较好的剂量依赖关系。结论体外土鳖虫水提物没有抗肿瘤活性,醇提物有较强的抗肿瘤作用,值得进一步研究其抑制机理。Objective To investigate antineoplasmic activity of extract of eupolyphaga on human gastric low-differentiation adenocarcinoma cell line BGC-823 in vitro. Methods The MTT assay has been developed for quantitative evaluation of the proliferation of BGC-823 of water extract and ethanol extract of eupolyphaga. The cytotoxic effect of ethanol extract of eupolyphaga was also assessed by MTT assay. Results Water extract of eupolyphaga has no cytotoxic effect on human gastric low-differentiation adenocarcinoma cell line BGC-823 in vitro, but ethanol extract of eupolyphaga has significant effect. The cytotoxic effect is the maximum at 48 hours and have a dose-dependent relation. Conclusion Ethanol extract of eupolyphaga has cytotoxic effect in vitro, while water extract hasn＇t. It is valuable to investigate the mechanism of cytotoxic effect.

关 键 词：土鳖虫 BGC-823细胞株 抗肿瘤活性 

分 类 号：R979.1[医药卫生—药品]