濒危植物岩黄连叶片中基因组DNA的提取及分析  

Isolation and Analysis of Genomic DNA from Leaves of Corydalis saxicola Bunting

作　　者：程华[1] 周蓬蓬[1] 余龙江[1] 胡琼月[1] 朱路[1] 刘智[1] 敖明章[1] 

机构地区：[1]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《时珍国医国药》2006年第9期1699-1700,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的获取高质量的岩黄连基因组DNA。方法用改进的SDS-CTAB法从岩黄连叶片中提取基因组DNA,通过琼脂糖凝胶电泳、紫外分光光度法、PCR检测其质量,并与传统的CTAB法比较。结果通过改进的SDS-CTAB法提取的DNA优于CTAB法提取的DNA,电泳条带清晰,纯度高,能较好的进行PCR。结论改进的SDS-CTAB法适合岩黄连基因组DNA的提取。Objective In order to obtain high quality genomic DNA of Corydalis saxicola Bunting, an effective method was established, Methods Two different methods,CTAB method and improved SDS-CTAB method, were applied to isolate genomic DNA from leaves of C. saxicola. The DNA obtained was analyzed by the means of agarose gel electrophoresis, spectrophotometer measurement and PCR. Results Higher purity DNA was obtained by improved SDS-CTAB method and it showed good response to PCR. Conclusion The improved SDS-CTAB method was favorable for the extraction of genomic DNA from C, saxicola.

关 键 词：岩黄连 基因组DNA SDS-CTAB法 

分 类 号：Q946.2[生物学—植物学]