大豆样品中DNA的提取与外源基因CaMV35S的PCR检测  

Extraction of Bean DNA and Detection of Transgenic CaMV35S by PCR Method

作　　者：尚智美[1] 高宏伟 王学亮[3] 孙伟[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,山东青岛266042 [2]青岛出入境检验检疫局,山东青岛266002 [3]菏泽学院化学系,山东菏泽274015

出　　处：《菏泽学院学报》2006年第2期73-75,共3页Journal of Heze University

基　　金：青岛市自然科学基金项目(04-2-JZ-114)

摘　　要：采用试剂盒从大豆样品中成功提取出DNA,对花椰菜花叶病毒(Cauliflower mosaic virus,CaMV)35S启动子进行了PCR扩增,并对扩增产物进行了电泳检测,建立了提取大豆DNA与CaMV35S启动子PCR检测的方法,对实验操作中的问题进行了探讨.The DNA was extracted from bean samples and the transgenic elements of cauliflower mosaic virus （CaMV） 35S promoter was amplified by polymerase chain reaction （PCR） method. The PCR products were detected by electrophoresis system. The method was stable and sensitive for the CaMV35S detection and the common problems in PCR procedure were discussed.

关 键 词：转基因大豆 PCR CaMV35S DNA 

分 类 号：Q788[生物学—分子生物学]