禽白血病病毒分离鉴定  

Isolation and Identification of Avian Leukosis Virus

作　　者：范书才[1] 李虹[1] 肖睿[1] 

机构地区：[1]中国兽医药品监察所,北京100081

出　　处：《中国兽药杂志》2006年第9期31-33,共3页Chinese Journal of Veterinary Drug

摘　　要：使用SPF鸡胚成纤维细胞从蛋用种鸡的病料中分离到一株病毒。经禽白血病病毒(ALV)p-27抗原ELISA检测、病毒培养、反转录-聚合酶链式反应(RT-PCR)鉴定、群特异性抗血清中和试验和动物回归试验等证明,该株病毒属于禽白血病病毒。A strain of virus was isolated from laying breeding chicken using primary chicken embryo fibroblasts （CEFs） freshly prepared from embryos （9 - 10 days old） of the line 0 leghorn chicken strain held as a specific pathogen-free （SPF） flock and identified to be the avian leukosis virus （ALV） by the assays such as detection of the isolate using ELISA with ALV p-27 Ag, virus propagation in CEFs, detection of viral genome by RT-PCR, neutralization of virus by subgroup-specific antiserum and infection of 1-day-old SPF chicken inoculated using the isolate.

关 键 词：禽白血病病毒 分离 鉴定 

分 类 号：S852.659.3[农业科学—基础兽医学]