毛细管电泳-化学发光法用于兔血中贝诺酯代谢物的分析  

Application of Capillary Electrophoresis with Chemiluminescence Detection in the Measurement of Benorilate Metabolites in Rabbit

作　　者：甘婷婷[1] 陈华[1] 夏之宁[1] 蒋雪梅[1] 张蓉[1] 辛龙涛[2] 喻彦林[1] 

机构地区：[1]重庆大学化学化工学院药学系,重庆400044 [2]太极集团有限公司,重庆400015

出　　处：《分析测试学报》2006年第5期66-68,72,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20375051);教育部跨世纪优秀人才培养计划基金资助项目(教技函[2001]3)

摘　　要：建立了一种用于药物在体内微量代谢物分析的毛细管电泳与化学发光在线联用方法，采用自制毛细管电泳-化学发光联用装置，对家兔血液中贝诺酯的活性代澍物扑热息痛进行了分析。考察了各种影响因素后，得到优化的电泳分离条件。在优化条件下，监测了贝诺酯灌胃后家免血液中扑热息痛的含量变化。扑热息痛含量在贝诺酯灌胃后4h达到峰值，扑热息痛的回收率为90％～115％，峰面积的RSD为2．1％-2．9％。本方法快速简单，检测灵敏度高，干扰小，适用于生物样品的分析。An analytical method based on on-line coupling of capillary electrophoresis with chemiluminescence detection was established to monitor the active metabolites of benorilate in rabbit plasma. The optimized condition in electrophoresis was determined after evaluating various controlling factors： Running buffer was 12 mmol · L^-1 borax and 4 mmol · L^-1 sodium hydroxide containing 5 mmol · L^-1 β-cyclodextrin and 5 mmol · L^-1 luminol, running voltage was 13 kV, the sample solution was injected 3 s with electrokinetic mode. Under the optimized conditions, the concentration of paracetamol in rabbit plasma in different time was determined. The linear range of paracetamol in rabbit plasma was about 0. 1 -5.0 mg · L^-1, with the determination limit of 3.81 × 10^-2 mg · L^-1 （signal-to-noise ratio is 3）. The average recoveries and RSD（n =4） for peak area were 90% -115% and 2. 1%-2.9% , respectively. The characteristics of this method are high speed, simply and sensitive, it is suitable for the analysis of biological samples, to determine the drug＇s concentration in plasma.

关 键 词：毛细管电泳 化学发光 药物代谢 贝诺酯 扑热息痛 

分 类 号：O657.8[理学—分析化学] TQ460.72[理学—化学]