微泡联合纤维蛋白原诱导超声栓塞肠系膜微血管的实验研究  

Experimental study of mesentery microvessel embolism induced by microbubbles combining with fibrinogen enhanced therapeutic ultrasound

作　　者：李秋颖[1] 刘政[1] 刘德英[1] 杨莉[1] 谭开彬[1] 付赤学[1] 刘平[1] 高云华[1] 

机构地区：[1]重庆第三军医大学新桥医院超声科,400037

出　　处：《临床超声医学杂志》2006年第8期449-451,共3页Journal of Clinical Ultrasound in Medicine

基　　金：国家自然基金项目(30470468);教育部留学回国人员科研启动基金

摘　　要：目的探讨携带纤维蛋白原的脂质微泡联合低强度治疗超声栓塞微血管的可行性。方法12只新西兰大白兔随机分为单纯治疗超声组、单纯纤维蛋白原微泡组、纤维蛋白原治疗超声组、纤维蛋白原微泡治疗超声组共4组进行实验。静脉注射携带纤维蛋白原的微泡,用频率为2MHz的低强度治疗超声照射,用体视显微镜观察,对比超声照射前后肠系膜微血管内血液流动情况;照射后经静脉推注伊文思蓝溶液,肉眼观察照射区域肠系膜微血管伊文思蓝灌注后染色情况。结果单纯治疗超声组、单纯纤维蛋白原微泡组、纤维蛋白原治疗超声组照射前后微血管内血液流动均通畅、迅速,伊文思蓝染色呈蓝色;纤维蛋白原微泡治疗超声组照射后,镜下见微血管内血液流动消失,血栓形成,伊文思蓝染色未见充填,仍呈红色。结论携带纤维蛋白原的微泡联合低强度治疗超声照射微血管可以栓塞肠系膜微血管,阻断局部血供。Objective To investigate the feasibility of mesentery microvaseular occlusion using therapeutic ultrasound combined with fibrinogen conjugated micmbubbles. Methods Twelve New Zealand white rabbits were divided into four groups at random for different therapeutic combinations. The therapeutic factors included fthrinogen conjugated microbubbles, therapeutic ultrasound （US） and fibrinogen solution. Blood flow of mesentery micmvessels were examined under a stereomicroscope before and after US radiation. Evans blue dye （EB） was injected intravenously to trace the circulation. Results Blood flow current of mesentery was smooth and rapid before and before in all of the rabhits and it remained smooth and rapid after therapies in the groups of the simple fibrinogen conjugated microbubbles, the simple therapeutic US and the fibrinogen plus therapeutic US. The mesentery micmvessels were blocked in the group of fibrinogen conjugated micmbubbles plus therapeutic US. EB staining was found in all of mesentery sectors except the insonated territory of the last group, which remained red. Conclusion This preliminary study indicated low intensity therapeutic US can block the mesentery micmvessels when combined with fibrinogen binded micmbubbles.

关 键 词：超声检查 造影剂 纤维蛋白原 治疗超声 肠系膜微血管 

分 类 号：R965.2[医药卫生—药理学]