恶性胶质瘤患者外周血淋巴细胞基因表达谱的研究  

Multiform peripheral blood lymphocyte gene expression spectrum in glioblastoma patients

作　　者：程迎新[1] 唐文渊[1] 

机构地区：[1]重庆医科大学附属第一医院神经外科,重庆400016

出　　处：《第三军医大学学报》2006年第16期1707-1709,共3页Journal of Third Military Medical University

摘　　要：目的运用基因芯片技术研究恶性胶质瘤患者和正常人外周血淋巴细胞基因表达差异,以探讨恶性胶质瘤患者免疫细胞的基因表达特点。方法应用含13824点cDNA的人类基因表达谱芯片分别检测23例恶性胶质瘤患者和40例正常外周血淋巴细胞基因表达差异。提取恶性胶质瘤患者和健康人淋巴细胞的mRNA,反转录制备标有不同荧光素的cDNA探针,将探针与芯片杂交后扫描荧光强度并数字化,用专业软件对检测结果进行分析从而筛选出差异性表达的基因。结果发现恶性胶质瘤患者淋巴细胞有96个基因呈现差异表达,其中上调表达的基因25个,下调表达的基因71个。结论恶性胶质瘤患者和正常人的外周血淋巴细胞基因表达存在差异。淋巴细胞DNA结合,转录功能异常和信号传导功能异常可能是恶性胶质瘤患者免疫功能有别于健康人的原因之一。Objective To study the difference of the peripheral blood lymphocyte gene expression in the glioblastoma patients and the normal subjects by the gene chip engineering technique. Methods The human gene expression spectrum chip containing 13 824 eDNA was used to detect the gene expression in the peripheral blood lymphocytes of 23 glioblastoma patients and 40 normal persons. The lymphocyte mRNA in the glioblastoma patients and healthy subjects was withdrawn. The eDNA probe with the different fluorescein was made by reverse transcription. The probe and the chip were hybridized. The fluorescence intensity was scanned and digitized, and gene expression was compared between the two groups. Results The peripheral blood lymphocytes in the glioblastoma patients had 96 genes to present the difference expression with that of normal subjects, ineluding 25 upregulated genes and 71 downregulated genes. Conclusion The gene expression in the peripheral blood lymphocytes of glioblastoma patients and normal subjects exists difference. The lymphocyte DNA union, the abnormal function of transcription and signal conduction are the possible reasons for the different immune function between the glioblastoma patients and the healthy subjects.

关 键 词：胶质瘤 基因芯片 淋巴细胞 血液 

分 类 号：R392.12[医药卫生—免疫学] R394.2[医药卫生—基础医学]