高渗和低渗造影剂对大鼠肾动脉内皮功能的影响及意义  

Effect of high osmolar and low osmolar contrast media on function of rat renal artery endothelium in vitro

作　　者：赵景宏[1] 黄岚[1] 王军平[2] 晋军[1] 宋耀明[1] 陈剑飞[1] 武晓静[1] 周健[1] 于世勇[1] 

机构地区：[1]第三军医大学新桥医院心血管内科,全军心血管内科中心,重庆400037 [2]第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2006年第16期1713-1715,共3页Journal of Third Military Medical University

摘　　要：目的探讨不同类型和剂量的造影剂对大鼠肾动脉内皮功能的影响和内皮细胞损伤情况,为造影剂肾病的发生及防治提供研究资料。方法肾动脉血管环在含不同剂量高渗造影剂泛影葡胺和低渗造影剂碘佛醇的IMDM培养液中孵育1h,Griess法和放免法分别检测培养液中一氧化氮(nitricoxide,NO)和内皮素(endothelin-1,ET-1)的含量,并用化学发光法检测血管内皮细胞乳酸脱氢酶(lactatedehydrogenase,LDH)的释放情况。结果肾动脉血管环在含泛影葡胺和碘佛醇的培养液中孵育1h后,NO分泌无明显变化,而ET-1分泌增加,且呈剂量依赖性。孵育4h后,泛影葡胺90μl/ml组肾动脉内皮LDH释放显著增加;碘佛醇各剂量组引起的LDH释放增加没有统计学意义。结论高渗造影剂泛影葡胺和低渗造影剂碘佛醇都可以影响肾动脉内皮功能,这一作用可能主要与ET-1分泌增加有关,而NO的分泌没有明显改变。泛影葡胺对肾动脉内皮功能的影响比碘佛醇严重,大剂量的泛影葡胺在一定时间内还可以导致孵育的肾动脉内皮细胞损伤。Objective To investigate the effect of different types and dosages of contrast media on the function and cell injury of rat renal artery endothelium. Methods Renal artery tings were isolated from SD rats and incubated in IMDM medium with 30, 60, 90 μl/ml Diatrizoate or Ioversol. The contents of NO and ET-1 were detected by Griess method and radioimmunoassay. The release of LDH was assessed by colotimetric method. Results After being incubated with Diatrizoate or Ioversol for 1 h, the renal artery endothelium did not show significant changes in the release of NO, but the release of ET-1 increased in a dose-dependent manner. When the renal artery endothelium was stimulated with Diatrizoate at dosage of 90 μl/ml for 4 h, the release of LDH was increased markedly. A slight, but insignificant, increased release of LDH induced by Ioversol was observed. Conclusion Both Diatrizoate and Ioversol can affect the function of renal artery endothelium by interfeting the secretion of ET-1, and the effects of Diatrizoate are more obvious than Ioversol. High-dose Diatrizoate can also induce renal artery endothelial cells injury.

关 键 词：造影剂 肾动脉内皮 一氧化氮 内皮素 乳酸脱氢酶 

分 类 号：R322.121[医药卫生—人体解剖和组织胚胎学] R322.61[医药卫生—基础医学]