红叶石楠“红罗宾”组培苗生根研究  

Studies on Rooting of Plantlet of Photinia fraseri "Red Robin" in vitro

作　　者：黄美娟[1] 黄海泉[1] 连芳青[2] 邓小梅[3] 

机构地区：[1]西南林学院园林学院,云南昆明650224 [2]江西农业大学园林与艺术学院,江西南昌330045 [3]江西省林业科学院,江西南昌330032

出　　处：《云南农业大学学报》2006年第5期565-570,575,共7页Journal of Yunnan Agricultural University

基　　金：西南林学院园林学院青年面上基金

摘　　要：对红叶石楠“红罗宾”(Photinia fraseri“Red Rob in”)离体培养苗生根的主要影响因子进行了系统的研究和分析。发现在NAA,IBA,IAA和ABT生长素中,NAA对红叶石楠的生根作用效果最好,最佳浓度为0.3 mg/L;在MS,1/2MS,B5,W h ite和M木(木本培养基)5种基本培养基中,M木对生根影响最大,为最佳生根基本培养基;同时发现,自然添加物E物质对红叶石楠生根有巨大的促进作用,在添加E物质后,红叶石楠根数量明显增多且粗壮、出根整齐而快,10 d内生根率达98.5%,比对照提高了30多个百分点,出苗时间提前了10多d。红叶石楠生根的最佳培养基为:M木+NAA 0.3 mg/L+E物质6.0 g/L。The principal factors contributed to rooting culture of Photiniafraseri ＂Red Robin＂ in vitro have been systematically studied and analyzed. The results showed that the effects of NAA （esp. the number, thickness and stockiness of root） were superior on adventitious root formation of Photiniafraseri to any other three tested auxins （named IBA, IAA and ABT respectively） , and its optimal concentration was 0. 3 mg/L. Among five basal media tested in this study （ including MS, 1/2 MS, B5, White and M,）, M, was better than other four kinds of media. Meanwhile, the natural additive substance E had a strong impact on its adventitious root formation. The roots of Photinia fraseri grew so fast and in order in the M, culture medium with substance E that its rooting rate had reached 98.5% within 10 d and much more 30 percentage than control, that its transplanting time was much shorter 10 d than control. The optimal rooting medium： M木 ＋ NAA 0. 3 mg/L ＋ Substance E 6 g/L.

关 键 词：红叶石楠“红罗宾” 组织培养 生根 E物质 

分 类 号：S686.035[农业科学—观赏园艺]