强心汤对实验性心肌肥厚大鼠血管紧张素Ⅱ及心肌超微结构的影响  

EFFECTS OF QIANGXIN DECOCTION ON ANGIOTENSIN Ⅱ AND MYOCARDIAL ULTRASTRUCTURAL CHANGES IN RATS WITH EXPERIMENTAL MYOCARDIAL HYPERTROPHY

作　　者：宁晓然[1] 王敬联[1] 郑伟[2] 陶杰梅[1] 于春英[2] 

机构地区：[1]河北省人民医院风湿免疫科,河北石家庄050051 [2]河北省人民医院中医科,河北石家庄050051

出　　处：《河北医科大学学报》2006年第5期327-330,341,共5页Journal of Hebei Medical University

基　　金：河北省科技厅科研基金(972761156D)

摘　　要：目的探讨中药强心汤对实验性心肌肥厚大鼠的心肌局部和血浆血管紧张素Ⅱ的影响。方法异丙肾上腺素皮下注射连续7 d建立大鼠心肌肥厚模型。30只大鼠随机分为对照组、模型组和造模后强心汤治疗组。造模第2 d起强心汤灌胃,连续用药12周,治疗12周后处死大鼠,测量各组大鼠左心室质量指数,放射免疫法测定血浆和心肌局部血管紧张素Ⅱ的水平,观察心肌超微结构。结果强心汤组与模型组比较,模型组血浆及心肌局部血管紧张素Ⅱ含量、左心室质量指数升高,与对照组比较差别有显著性意义(P<0.05);强心汤组血浆及心肌局部血管紧张素Ⅱ含量、左心室质量指数明显下降(P<0.05,P<0.01),与模型组比较差别有显著性意义(P<0.05);心肌超微结构损伤程度明显减轻。结论强心汤可通过抑制心肌局部血管紧张素Ⅱ含量和改善肥厚心肌细胞超微结构发挥逆转心肌肥厚的作用。Objective To investigate the effects of Qiangxin decoction on myocardial tissue and plasma levels of angiotensin Ⅱ in rats with experimental left ventricular hypertrophy. Methods Thirty rats were divided into normal control group, model group and Qiangxin group randomly, with ten rats in each group. Myocardial hypertrophy model of rats were established by subcataneus injection of isoproterenol （5 mg/kg ·d） for 7 days. Rats were given Qiangxin decoction by gastric gavage for 12 weeks in Qiangxin group. Rats were killed by decapitation after 12 weeks, and the myocardial indexes（heart weight/body weight, HW/BW, and left ventricular mass index,LVMI） were measured. Myocardial and plasma angiotensin Ⅱ levels were measured in all groups by radioimmunoassay. Myocardial ultrastructure were observed with transmission electron microscope. Results LVMI level in the model group was obviously higher than that of the control group （ P〈0.05）, while in Qiangxin group, LVMI was decreased significantly as compared with the model group. The myocardial and plasma angiotensin Ⅱlevels in the model group were obviously higher than those of the control group（ P 〈0.05）,while that in Qiangxin group were decreased significantly（ P 〈0.01, P d0.05）. The degree of injury on ultrastructures of myocardial cell in model group was severer significantly than that in Qiangxin group. Conclusion Qiangxin decoction could reduce the left ventricular hypertrophy by decreasing of angiotensin Ⅱ levels in cardial tissue and plasma, and could protect the ultrastructures of myocardial cell.

关 键 词：心肌病 肥厚性 强心汤 血管紧张素Ⅱ 大鼠 

分 类 号：R285.5[医药卫生—中药学]