杀菌剂啶菌噁唑的高效液相色谱分析  

Determination of Fungicide SYP-Z048 by High Performance Liquid Chromatograph

作　　者：韩平[1] 刘鹏飞[1] 司乃国[2] 刘西莉[1] 

机构地区：[1]中国农业大学植物病理学系,北京100094 [2]沈阳化工研究院,辽宁沈阳110021

出　　处：《农药学学报》2006年第3期288-290,共3页Chinese Journal of Pesticide Science

摘　　要：建立了一种用反相高效液相色谱测定啶菌噁唑的定量分析方法。采用C18ODS色谱柱，以甲醇-水（75：25，体积比）作为流动相，流速1．0mL／min，检测波长220nm。啶菌噁唑色谱图显示两个吸收峰，分别为两个同分异构体Z体和E体。在400～700μg／mL浓度范围内，标准溶液浓度和吸收峰面积之间有良好的线性关系，回归方程为Y=4．8467X＋232．85，相关系数r为0．9992；对原药溶液及其制剂溶液测定的重复性SR分别为0．0040和0．0047。该方法准确、精密，适用于啶菌噁唑的定量分析。A method for quantitative analysis of fungicide SYP-Z048 [5- （4-chlorophenyl） -2,3-dimeth- yl-3-（ pyridine-3-yl ）-oxazoline]was developed by a reversed-phase high performance liquid chromatograph. The chromatogram of SYP-Z048 showed two absorbance peak by a C18ODS column, which belonged to Z and E, respectively. The mobile phase consisted of methanol and water （V/V =75：25 ） and the flow rate was 1 mL/min. The wavelength of detector was 220 nm. When concentration of standard solvent ranged from 400 - 700 μg/mL, there was good linear relationship between concentration of standard solvent and area of absorbed peak. The regression equation was Y = 4.846 7X ＋ 232.85, and the correlation coefficient of the standard curve was 0.999 2. The repeatability SR of technical SYP-Z048 and its formulation were 0. 004 0, 0. 004 7, respectively. The method was oroved to be accurate and orecise to successfully measure the SYP-Z048.

关 键 词：啶菌噁唑 高效液相色谱 分析方法 

分 类 号：O657.72[理学—分析化学]