光纤型PDMS电泳芯片的制作与研究  

Fabrication and Study on PDMS Electrophoresis Chip with Fiber

作　　者：苏波[1] 崔大付[1] 刘长春[1] 陈兴[1] 孙建海[1] 

机构地区：[1]中国科学院电子学研究所传感技术国家重点实验室,北京100080

出　　处：《测试技术学报》2006年第5期402-406,共5页Journal of Test and Measurement Technology

基　　金：国家自然科学基金资助项目(20299030;60341005)

摘　　要：介绍了一种光纤型PDM S电泳芯片,该芯片主要由多模光纤,PDM S盖片和基片组成.通过浇注PDM S的方法制成电泳芯片,盖片上的微流控沟道和光纤沟道的深度分别为50μm和90μm,基片上的光纤沟道的深度为40μm,实现了微流控沟道中心和光纤的中心在同一个平面上.用蓝色发光二极管作为激发光源,以异硫氰酸酯荧光素为分离物质,在自制的电泳仪上对该芯片进行测试,最小检测浓度达到1.1×10-7m o l/L,信噪比S/N=3,在1.8×10-7m o l/L^4×10-5m o l/L范围内相关系数r=0.996,结果证实了该芯片的可行性.The fabrication of PDMS electrophoresis chip with fiber is reported which consists of multimode optical fiber, a PDMS wafer with microcharmel network and PDMS cover. The electrophoresis chip was fabricated by pouring. The depths of microchannel and fiber channel in the PDMS cover were 50/am and 90/am respectively, and the depth of fiber channel in the PDMS wafer was 40/am, so the center of microfluidic channel could be located on the same plane with the fiber center easily. The detection experiment was performed by the homemade electrophoresis instrument with blue LED as exciting light source and FITC as separation substance. The minimum detectable concentration Of 1. 1 × 10^-7 mol/L（S/N=3） was obtained and the relevant coefficient in the range of 1.8× 10^-7 mol/L -4× 10^-5 mol/L was 0. 996. The results indicate that the chip is feasible in electrophoresis separation.

关 键 词：光纤 PDMS 电泳芯片 荧光检测 

分 类 号：O657[理学—分析化学]