萘哌地尔衍生物BWYJ抗前列腺增生的作用  

Effect of naftopidil' ramification-BWYJ on benign prostatic hyperplasia in mice and rats

作　　者：于爽[1] 余丽梅[2] 袁牧[3] 于泓[4] 吴芹[2] 石京山[2] 

机构地区：[1]郑州大学基础医学院药理学教研室 [2]遵义医学院药理学教研室 [3]贵州省中科院天然产物重点实验室 [4]遵义医学院附属医院泌尿内科

出　　处：《中国临床药理学与治疗学》2006年第8期873-877,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(№30060087);贵州省科技基金重点项目(计[2002(3014)])

摘　　要：目的观察萘哌地尔衍生物BWYJ对前列腺增生模型的作用。方法采用激素法建立去势大鼠和未去势小鼠前列腺增生模型,通过小鼠前列腺湿重,计算前列腺指数。光镜及透射电镜下,分别观察小鼠前列腺组织形态学及超微结构变化;TUNEL法检测BWYJ对大鼠前列腺细胞凋亡的影响。结果BWYJ5、10、20mg·kg-1组均可降低BPH小鼠前列腺湿重指数(P<0.05),光镜及电镜结果表明,BWYJ5、10、20mg·kg-1组均可抑制小鼠组织结构增生性变化,且BWYJ10、20mg·kg-1组使腺腔直径、腺体表面积变小(P<0.05)。TUNEL检测发现,大鼠前列腺凋亡细胞检出率较低,与模型组比较,BWYJ各剂量组差异无统计学意义(P>0.05)。结论BWYJ具有抗小鼠及大鼠良性前列腺增生作用。AIM：To observe the effect of naftopidilderivant BWYJ on benign prostatic hyperplasia （BPH） induced by testosterone propionate in castrated rats and uncastrated mice. METHODS： Castrated rats and uncastrated mice were injected subcutaneously testosterone propionate to induce BPH. The effect of BWYJ on mice with BPH was obtained by the prostatic wet weight and wet weight index. As well morphological changes of prostate were observed through light microscope and electron microscope, and were semi-quantified by image analysis system. The apoptotsis of prostatic cells in rat was detected by TUNEL assay. RESULTS：BWYJ decreased the prostate wet weight index （ P 〈 0.05）. BWYJ inhibited histomorphological changes of BPH induced by testosterone propionate in uncastrated mice. Few apoptosis cell was detected by TUNEL assay in BWYJ-treated groups compared with model group （ P 〉 0.05 ）. CONCLUSION： BWYJ inhibits BPH induced by testosterone propionate in KM mice and SD rat.

关 键 词：萘哌地尔衍生物 BWYJ 前列腺增生 组织形态学 电镜 细胞凋亡 

分 类 号：R972.4[医药卫生—药品] R965.1[医药卫生—药学]