海带(Laminaria japonica)幼孢子体生长和光合作用的N需求  

Nitrogen Requirement of Growth and Photosynthesis in the Juvenile Sporophyte of Laminaria japonica

作　　者：吴荣军[1] 朱明远[2] 李瑞香[2] 张学雷[2] 郑有飞[1] 孙丕喜[2] Jean-Marc Deslous-Paoli Isabelle Auby 

机构地区：[1]南京信息工程大学环境科学与工程系,江苏南京210044 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]法国海洋开发研究院生态和海水养殖研究中心

出　　处：《海洋通报》2006年第5期36-42,共7页Marine Science Bulletin

基　　金：科技部国际合作重点项目(2002CB714008);欧盟项目(INCO-CT-2004-510706);南京信息工程大学科研基金(Y307)资助

摘　　要：根据室内和围隔实验中海带幼孢子体在不同硝态氮浓度下的生长情况和光合作用速率(Pmax),分析了幼孢子体的N需求,得到其最大生长率(μm)为0.12d-1,维持生存的最低组织N含量(NQ)为16.8μg/mgDW,以最大生长率生长所必需组织N的临界值(NC)为20.4μg/mgDW,每天以最大速率生长的N需求(Nreq)为2.45μg/mgDWd-1。同时,不同处理组的初始NO3-N浓度越高,海带幼孢子体吸收速率和组织N的累加速率越高,且呈明显的线性相关关系(R2分别为0.8393和0.7793,P<0.05)。现场围隔实验中,叶绿素a含量(R2=0.7907,P<0.05)和组织N含量(R2=0.9147,P<0.01)与Pmax也呈明显的线性相关关系。同时,根据海带幼孢子体N的需求和营养吸收状况,分析认为,海带幼孢子体存在受到N限制的风险,但凭其营养吸收能力有适应N限制的能力。还根据海带的这种生理特征,探讨了大型海藻的养殖对富营养化海水的生态调控。According to the growth and photosynthetic capacity of juvenile sporophyte of Lamina ria japonicaunder different nitrate concentrations in laboratory and mesocosm experiments, the N requirements of juvenile sporophyte were analyzed, indicating that the maximum growth rate （ μm ） is 0.12 d^-1, the subsistence N quota is （NQ） 16.8 μg/mgDW, the critical N concentration to sustain growth at maximum rate （Ac） is 20.4 μg/mgDW and N requirements for the maximum growth rate （Nreq） is 2.45 μg/mgDWd^-1. Simultaneously, the uptake rate of nitrate and accumulative rate of tissue N have a greater linearly relative response to the initial nitrate concentration respectively. Chlorophyll a content and tissue N content have a greater linear relative response to Pmax in mesocosm experiment too. Accordingly, the juvenile sporophyte of L. japonica faces a higher risk of N limitation concluded by N requirement and nutrients status of tissue, but possesses the ability of adaptation to N limitation evidenced by a higher uptake rate of nutrients. At the same time, the paper also discussed ecological regulation and control utilizing aquacultural macroalgae to nutrient stress and eutrophication.

关 键 词：海带 生长 光合作用 N需求 

分 类 号：P745[天文地球—海洋生物学]