加拿大披碱草与2种国产披碱草杂种F_1愈伤组织诱导及植株再生研究  

Callus Induction and Plant Regeneration in F_1 Hybrids of Elymus canadensis×E.dahuricus and E.cylindricus

作　　者：马艳红[1] 于卓[1] 李小雷[1] 李造哲[1] 

机构地区：[1]内蒙古农业大学农学院,呼和浩特010019

出　　处：《西北植物学报》2006年第9期1888-1892,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30560100);内蒙古自然科学基金(200607010505)

摘　　要：对加拿大披碱草×披碱草F1和加拿大披碱草×圆柱披碱草F1的幼穗外植体愈伤组织诱导及植株的再生进行了研究.试验筛选出2个杂交种愈伤组织诱导的最适培养基均为:MS+400 mg/L CH+3.0 mg/L 2,4-D;最适继代培养基为MS+400 mg/L CH+1.0 mg/L 6-BA+0.5 mg/L 2,4-D;最适幼苗分化培养基为:MS+400 mg/LCH+3.0 mg/L 6-BA+0.5 mg/L NAA;最适生根培养基为:MS+0.5 mg/L NAA+0.5 mg/L IBA.这一组培体系的建立,为2个种间杂交种的种质保存及利用奠定了基础.The study investigated callus induction and plant regeneration of young spikes in hybrid F1 of Elymus canadensis × E. dahuricus and E. cylindricus. It found that for the two hybrids the optimal callus induction mediums were MS＋400 mg/L CH ＋3.0 mg/L 2,4-D,the optimal secondary mediums were MS ＋400 mg/L CH ＋1.0 mg/L 6-BA＋ 0.5 mg/L 2,4-D, the optimal mediums of plantlet differentiation were MS＋400 mg/1. CH ＋3.0 mg/L 6-BA＋ 0.5 mg/L NAA and the optimal mediums for rooting were MS＋ 0.5 mg/L NAA＋0.5 mg/L IBA. This tissue culture system laid a foundation for germplasm conser- vation and exploitation of the two interspecific hybrids.

关 键 词：加拿大披碱草 披碱草 圆柱披碱草 种间杂种F1 愈伤组织诱导 植株再生 

分 类 号：Q813.12[生物学—生物工程]