利用微卫星标记鉴定北方杂交粳稻种子纯度的研究  

Studies on Identification of Seed Purity of Japonica Hybrid Rice in North China Using Microsatellite Markers

作　　者：马秀芳[1] 华泽田 隋国民 郝宪彬 吕桂兰 王铮 

机构地区：[1]沈阳农业大学,辽宁沈阳110161 [2]辽宁省稻作研究所,辽宁沈阳110101

出　　处：《杂交水稻》2006年第5期56-58,63,共4页Hybrid Rice

基　　金：辽宁省科技基金(2040564);国家农业部948项目;水稻全球分子育种计划项目(30040025)

摘　　要：利用48对SSR引物对7个北方杂交粳稻组合的杂种F1与其父母本间的多态性进行了筛选，每个组合获得4-11对多态性好的特异引物，利用特异性引物可将杂交种与其相应的不育系、保持系和恢复系分开，说明SSR标记可应用于北方杂交梗稻种子纯度鉴定，具有较高的实用价值。Seven three-line japonica hybrid rice combinations in North China and their parents were used to screen their polymorphic patterns with microsatellite markers. Out of 48 frame primer pairs, 4 - 11 pairs showed polymorphic patterns in each of the tested combinations, by which the A, B and R lines can be well identified from their F1 hybrids, indicating that these SSR markers can be practically used to identify the seed purity of japonica hybrid rice in North China.

关 键 词：杂交梗稻 微卫星标记 种子纯度鉴定 

分 类 号：S511.01[农业科学—作物学]