自由基促进猪内皮细胞c－sis　mRNA／PDGF－B链蛋白表达的实验研究  

An Experimental Study of Promoting Effect of Free Radicals on the Expression of c-sis mRNA/PDGF-B Chain Protein in Endothelial Cells

作　　者：王宏伟[1] 赵华月[1] 向世勤[1] 

机构地区：[1]武汉同济医科大学附属同济医院内科

出　　处：《同济医科大学学报》1996年第5期338-340,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金

摘　　要：采用免疫组织化学和原位杂交方法，研究自由基（ＦＲ）对猪主动脉内皮细胞（ＥＣ）的ｃ－ｓｉｓｍＲＮＡ及血小板源生长因子（ＰＤＧＦ）Ｂ链蛋白表达的影响。结果显示，ＦＲ促进ｃ－ｓｉｓｍＲＮＡ和ＰＤＧＦ－Ｂ链蛋白的表达。ＥＣ暴露于ＦＲ２０ｍｉｎ，ｃ－ｓｉｓｍＲＮＡ和ＰＤＧＦ－Ｂ链蛋白的表达较对照组分别增加１．０和２．２倍。提示，ＦＲ损伤ＥＣ所诱导的ＰＤＧＦ合成增多在动脉粥样硬化的发病过程中可能起重要作用。The effects of free radicals (FR) on the expression of c-sis mRNA and platelet-derived growth factor(PDGF)B chain protein in porcine aortic endothelial cells(EC)were studied with immunohistochemistry and in situ hybridization metheds.The results showed that FR enhanced the c-sis mRNA and PDGF-B chain protein expression signicantly.Exposing EC to FR releasing system for 20 min, the expressions of c-sis mRNA and PDGF-B chain protein were increased 1.0 and 2.2 times respectively as compared with that of the control group.These findings suggest that the synthesis of PDGF may be increased through damaging EC by FR, which in turn may play an important role in the pathogenesis of atherosclerosis.

关 键 词：内皮细胞 自由基 血小板源 生长因子 原位杂交 

分 类 号：R541.402[医药卫生—心血管疾病] R364.32[医药卫生—内科学]