除草剂草甘膦对大鼠肝微粒体酶及脂质过氧化作用的影响  

Effects of Glyphosate on Hepatic Microsomal Enzymes and Lipid Peroxidation in Ratso

作　　者：邬惠琼[1] 毛新良[1] 严红[1] 王新刚[1] 柴莲花[1] 

机构地区：[1]武汉同济医科大学公共卫生学院环境毒理学研究室

出　　处：《同济医科大学学报》1996年第5期358-361,共4页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金

摘　　要：通过体内和体外试验，研究了草甘膦对大鼠肝微粒体酶、脂质过氧化作用及还原型谷胱甘肽的影响。体内试验结果表明：草甘膦能显著诱导增加微粒体细胞色素Ｐ（４５０）含量，与对照组比较［ｘ±ｓ（下同）分别为（０．６８±０．１２）、（０．５９±０．０９）μｍｏｌ／ｇ］具有显著意义（Ｐ＜０．０５）。并且可使还原型谷胱甘肽的含量显著增加，与对照组比较［分别为（１２８１．５±７４．０）、（１１７８．９±２２．０）μｇ／ｇ］具有极显著意义（Ｐ＜０．０１）。体外试验结果表明：草甘膦在１０（－８）～１０（－４）ｍｏｌ／Ｌ浓度范围内对细胞色素Ｐ（４５０）无明显影响，但能显著抑制脂质过氧化作用，在１０（－７）～１０（－４）ｍｏｌ／Ｌ时可增加还原型谷胱甘肽的含量，且呈明显剂量效应关系。Glyphosate is a broad spectrum, pestmergence and widely-ultilized herbicide.The effects of glyphosate on hepatic microsomal enzymes,lipid peroxidation and the content of glutathione in rats were studied in vivo and in vitro.The results in vivo indicated that Glyphosate dramatically increased the content of cytochrome P(450).Averages and standard deviations for treatment group and control group were(0.68±0.12)and(0.59±0.09)μmol/g, respectively.The difference in average was statisticslly significant at P<0.05.Glyphosate had a minor effect on the content of cytochrome b5 with an induction of activity of Aniline hydroxylase.The level of glutathione was raised significantly.At P<0.01 level,the difference in average was statistically significant. Averages and standard deviations for treatment group and control group were(1 281.5±74.0) and(1 178.9±22.0)μg/g, respectively.MDA(the product of LPO) was changed moderately by glyphosate.The test in vitro estimated that the effects on the content of hemoprotein(cytochrome P(450)and b5)and on the activity of Aniline hydroxylase were moderate or not observed in the range of 10(-8)￣10(-4)mol/L;whereas in this range of concentration lipid peroxidation was inhibited and the level of glutathione was increased dramatically, showing an obvious dose-effect relationship.

关 键 词：草甘膦 微粒体 肝 过氧化脂质 还原型 谷胱甘肽 

分 类 号：R995[医药卫生—毒理学] R361.2[医药卫生—药学]